Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
复制标题
拟南芥子叶异位血管诱导用于韧皮部分化的序列分析
DOI：
10.1007/978-1-4939-8657-6_10
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fukuda Hiroo
中科院分区：
文献类型：
--
作者：
Nurani Alif Meem;Kondo Yuki;Fukuda Hiroo
维管系统在许多陆生植物中对水分和营养物质的运输以及提供机械支持至关重要。植物转录因子在调节激素和肽信号通路下游的维管发育中起核心作用。特别是，细胞培养系统有助于分离木质部分化的关键转录因子。然而，在模式植物拟南芥中还没有有效的系统来模拟韧皮部的分化，阻碍了韧皮部相关转录因子的鉴定。我们最近建立了拟南芥叶片维管细胞诱导培养系统（VISUAL），该系统在拟南芥子叶中同时产生木质部和韧皮部细胞。该系统可用于进一步研究形成层细胞向木质部和韧皮部细胞双向分化的机制。在这里，我们报告了使用VISUAL的显微镜，遗传和分子分析方法，这可以帮助解密调节血管细胞分化的转录网络。
Vascular system is vital for the transport of water and nutrients as well as for providing mechanical support in many land plants. Plant transcription factors play a central role in regulating vascular development downstream of hormones and peptide signaling pathways. Particularly, cell culture systems have contributed to isolating such key transcription factors for xylem differentiation. However, there had been no efficient systems that can mimic phloem differentiation in the model plantArabidopsis, preventing the identification of phloem-related transcription factors. We have recently establishedVascular cellInduction cultureSystemUsingArabidopsisLeaves (VISUAL), which concomitantly generates both xylem and phloem cells in the cotyledon ofArabidopsis. This system can be used to take a closer look at the bi-directional differentiation mechanism of (pro)cambial cells into xylem and phloem cells. Here, we report the methods of microscopic, genetic, and molecular analysis using VISUAL, which can help in decrypting the transcriptional networks that regulate vascular cell differentiation.