IGG2A MONOCLONAL-ANTIBODIES INHIBIT HUMAN-TUMOR GROWTH THROUGH INTERACTION WITH EFFECTOR-CELLS
IGG2A MONOCLONAL-ANTIBODIES INHIBIT HUMAN-TUMOR GROWTH THROUGH INTERACTION WITH EFFECTOR-CELLS
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DOI：
10.1073/pnas.79.15.4761
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
HERLYN, D;KOPROWSKI, H
IgG2a同型单克隆抗体特异性抑制人肿瘤在裸鼠体内的生长。其他同型的单克隆抗体（23）无肿瘤杀伤反应性。裸鼠补体缺失对单克隆抗体的抑瘤作用无明显影响。T细胞和杀伤细胞作为裸鼠单克隆抗体效应介质的作用几乎被排除。巨噬细胞被强烈认为是效应细胞，因为二氧化硅处理可以消除单克隆抗体的杀瘤作用。IgG2a单克隆抗体依赖的巨噬细胞介导的细胞毒性实验在培养的人肿瘤细胞中导致肿瘤细胞特异性溶解。
Monoclonal antibodies of IgG2a isotype specifically inhibited growth of human tumors in nude mice. Monoclonal antibodies (23) of other isotypes showed no tumoricidal reactivity. Complement depletion of nude mice had no effect on tumor suppression by monoclonal antibody. The role of T and killer cells as mediators of the monoclonal antibody effect in nude mice was virually excluded. Macrophages were strongly incriminated as effector cells because silica treatment of nude mice abolished the tumoricidal effect of monoclonal antibody. IgG2a monoclonal antibody-dependent macrophage-mediated cytotoxicity assays with human tumor cells in culture resulted in specific lysis of tumor cells.