Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization.

Sequence-independent amplification and labeling of yeast artificial chromosomes for fluorescence in situ hybridization.
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DOI：
10.1159/000133612
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发表时间：
1994
期刊：
Cytogenetics and cell genetics
影响因子：
--
通讯作者：
S. Bohlander;R. Espinosa;A. Fernald;J. Rowley;M. Beau;M. Diaz
S. Bohlander;R. Espinosa;A. Fernald;J. Rowley;M. Beau;M. Diaz
中科院分区：
其他
文献类型：
--
作者：
S. Bohlander;R. Espinosa;A. Fernald;J. Rowley;M. Beau;M. Diaz

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我们开发了一种方法，基于脉冲场凝胶电泳和快速序列无关扩增程序 (SIA) 分离 YAC，可以从酵母人工染色体 (YAC) 可靠地构建高质量 FISH 探针。这些探针可用于定位中期染色体上的 YAC，也可高效定位间期细胞核中的 YAC。
We have developed a method that allows reliable construction of high quality FISH probes from yeast artificial chromosomes (YACs) based on the separation of YACs by pulse-field gel electrophoresis and a rapid sequence-independent amplification procedure (SIA). These probes can be used to localize YACs on metaphase chromosomes and also with high efficiency, in interphase nuclei.