NF-κB activity and iNOS expression in monocytes from internal jugular blood of migraine without aura patients during attacks
NF-κB activity and iNOS expression in monocytes from internal jugular blood of migraine without aura patients during attacks
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DOI：
10.1111/j.1468-2982.2006.01164.x
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发表时间：
2006-09-01
期刊：
影响因子：
4.9
通讯作者：
Calabresi, P.
中科院分区：
文献类型：
--
作者：
Sarchielli, P.;Floridi, A.;Calabresi, P.
本研究采用凝胶迁移率分析法研究了核因子-κ B（NF-κ B）活性，并采用Western印迹法分析了7例无先兆偏头痛患者发作期颈内静脉血中单核细胞I κ B α表达。诱导型一氧化氮合酶（iNOS）的表达也通过逆转录聚合酶链反应进行了评估。NF-κ B活性在发作后2 h达到峰值。这伴随着I κ B α表达的短暂降低。iNOS表达在4 h明显上调，6 h维持上调，至发作结束时下调。这些发现证实了暂时性延迟性炎症的假设，如动物模型所示，并表明在治疗偏头痛中使用靶向NF-κ B转录的治疗方法的可能性。
This study investigated nuclear factor-kappa B (NF-kappa B) activity by electrophoresis mobility gel shift assay and I kappa B alpha expression by Western blot analysis in monocytes obtained from serial samples of internal jugular venous blood taken from seven migraine patients without aura during attacks. Inducible nitric oxide synthase (iNOS) expression was also assessed by reverse transcription-polymerase chain reaction. An increase in NF-kappa B activity peaked 2 h after attack onset. This was accompanied by a transient reduction in I kappa B alpha expression. Up-regulation of iNOS was evident at 4 h, maintained at 6 h and reduced at the end of the attack. These findings substantiate the hypothesis of transitory delayed inflammation, as suggested by the animal model, and suggest the possibility of using therapeutic approaches to target NF-kappa B transcription in the treatment of migraine.