Mold Fabrication and Biological Assessment of Porous DNA-Chitosan Complexes
Mold Fabrication and Biological Assessment of Porous DNA-Chitosan Complexes
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DOI：
10.1002/jbm.b.31451
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发表时间：
2009-11-01
影响因子：
3.4
通讯作者：
Okahata, Yoshio
中科院分区：
文献类型：
--
作者：
Fukushima, Tadao;Ohno, Jun;Okahata, Yoshio
先前的研究表明，由天然DNA和壳聚糖在水溶液中反应制备的DNA-壳聚糖复合物具有适合细胞接种的孔隙率，无毒，并且仅引起轻微的软组织反应。这种简单易行的多孔DNA-壳聚糖复合物的制备方法作为支架材料提供了广泛的应用。本研究评价了用PBS溶液冲洗是否可以在模具中构建DNA-壳聚糖复合物以及大鼠软组织对所构建的DNA-壳聚糖复合物的组织病理学反应。将冷冻干燥后的DNA-壳聚糖复合物粉末与蒸馏水混合，制备DNA-壳聚糖复合物糊剂。DNA-壳聚糖复合物盘可以通过用PBS缓冲液冲洗并随后在模具中冷冻干燥DNA-壳聚糖复合物糊状物来制备。因此，DNA-壳聚糖复合物在组织工程中具有广泛的应用前景。盘的孔隙率为85%，并且在DNA-壳聚糖复合物（制造前）和制造的DNA-壳聚糖盘中可见许多孔。组织中复合盘的值逐渐减少，但仍有60%的复合盘保留在组织中。总之，开发了一种简单的制备DNA-壳聚糖复合物多孔盘的方法。结果表明，该方法可以延缓DNA-壳聚糖复合物的生物降解，且在体内没有严重的组织反应。DNA-壳聚糖复合物是一种很有前途的组织工程支架材料，在组织工程中具有广泛的应用前景。(C)2009 Wiley Periodicals，Inc.生物医学材料研究杂志B部分：应用生物材料91 B：746-754，2009
A previous study revealed that DNA-chitosan complex prepared from the reaction between native DNA and chitosan in aqueous solution has suitable porosity for cell seeding, is nontoxic, and causes only a mild soft-tissue response. This simple and easy fabrication method for porous DNA-chitosan complex provides for a wide variety of applications as a scaffold material. The present study evaluated whether rinsing with PBS solution can fabricate DNA-chitosan complex in a mold and the histopathological responses of rat soft tissues to fabricated DNA-chitosan complexes. DNA-chitosan complex paste was prepared by mixing distilled water and freeze-dried water-rinsed DNA-chitosan complex powder. A DNA-chitosan complex disk could be fabricated by rinsing with PBS buffer and subsequently freeze-drying the DNA-chitosan complex paste in the mold. Thus, a wide range of applications of DNA-chitosan complex for tissue engineering can be anticipated using the present easy fabrication method. The porosity of the disk was 85%, and many pores were visible in the DNA-chitosan complex (before fabrication) and in the fabricated DNA-chitosan disk. The values of the complex disks gradually reduced in the tissues although 60% of disks remained in the tissues. In conclusion, an easy fabrication method for making porous DNA-chitosan complex disks was developed. It was found that the fabrication method can delay the biodegradation of the DNA-chitosan complex disk without serious tissue responses in vivo. DNA-chitosan complex is promising as a scaffold material, and a wide range of applications of DNA-chitosan complex for tissue engineering are anticipated. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 746-754, 2009