A new mtDNA COI gene lineage closely related to Anopheles janconnae of the Albitarsis complex in the Caribbean region of Colombia
A new mtDNA COI gene lineage closely related to Anopheles janconnae of the Albitarsis complex in the Caribbean region of Colombia
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DOI：
10.1590/s0074-02762010000800011
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发表时间：
2010-12-01
期刊：
影响因子：
--
通讯作者：
Correa, Margarita M
中科院分区：
文献类型：
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作者：
Gutiérrez, Lina A;Orrego, Lina M;Correa, Margarita M
对分类状态和媒介分布的理解对于控制疟疾至关重要。先前的系统发育分析支持了六种新热带疟疾载体蚊（Nyssorhynchus）Albitarsis S.L.的描述。 （双翅目：Culicidae）：An。甲状腺素，肛门deaneorum，Anopheles marajoara，Anopheles oryzalimnetes，Anopheles Janconnae和An。 Albitarsis F.评估An的分类状态。 Albitarsis S.L.在哥伦比亚加勒比地区各个地方收集的蚊子，使用完整的线粒体DNA细胞色素氧化酶I（COI）基因，核糖体DNA（rDNA）内部转录的间隔2（ITS2）区域和部分核DNA白色基因序列分析了标本。 COI基因序列的系统发育分析检测到与An密切相关的新谱系。在哥伦比亚加勒比地区的Janconnae与该综合大楼的其他成员确定了其位置。但是，ITS2和白色基因序列缺乏足够的分辨率来支持与AN密切相关的新谱系。 Janconnae或An。 Janconnae进化枝。这种新谱系在哥伦比亚疟疾传播中的可能参与仍然未知，但其系统发育接近。 Janconnae与巴西当地的疟疾传播有关，令人着迷。
An understanding of the taxonomic status and vector distribution of anophelines is crucial in controlling malaria. Previous phylogenetic analyses have supported the description of six species of the Neotropical malaria vector Anopheles (Nyssorhynchus) albitarsis s.l. (Diptera: Culicidae): An. albitarsis, Anopheles deaneorum, Anopheles marajoara, Anopheles oryzalimnetes, Anopheles janconnae and An. albitarsis F. To evaluate the taxonomic status of An. albitarsis s.l. mosquitoes collected in various localities in the Colombian Caribbean region, specimens were analyzed using the complete mitochondrial DNA cytochrome oxidase I (COI) gene, the ribosomal DNA (rDNA) internal transcribed spacer 2 (ITS2) region and partial nuclear DNA white gene sequences. Phylogenetic analyses of the COI gene sequences detected a new lineage closely related to An. janconnae in the Caribbean region of Colombia and determined its position relative to the other members of the complex. However, the ITS2 and white gene sequences lacked sufficient resolution to support a new lineage closely related to An. janconnae or the An. janconnae clade. The possible involvement of this new lineage in malaria transmission in Colombia remains unknown, but its phylogenetic closeness to An. janconnae, which has been implicated in local malaria transmission in Brazil, is intriguing.