Response of microRNAs to cold treatment in the young spikes of common wheat.
Response of microRNAs to cold treatment in the young spikes of common wheat.
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普通小麦幼穗中 microRNA 对冷处理的响应
DOI：
10.1186/s12864-017-3556-2
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发表时间：
2017-02-28
期刊：
影响因子：
4.4
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Song G;Zhang R;Zhang S;Li Y;Gao J;Han X;Chen M;Wang J;Li W;Li G
研究背景MicroRNAs（miRNAs）是一类非编码小分子RNA，通过调控靶基因在生物和非生物胁迫中发挥重要作用。普通小麦春季霜冻灾害频繁发生，特别是在花器官分化早期低温发生时，会造成严重的产量损失。到目前为止，小RNA的作用在小麦应对霜冻stress.ResultsWe报告在这里的小RNA转录组的测序与冷胁迫处理的幼穗和与对照的比较分析。共鉴定出105个家族的192个保守miRNAs和9个新的miRNAs。其中，34个保守的和5个新的miRNAs在冷应激样品和对照之间差异表达。通过定量真实的时间聚合酶链反应（qRT-PCR）进一步验证18个miRNAs的表达模式。此外，近一半的miRNAs是交叉诱导的生物和非生物胁迫相比，以前发表的工作。通过degradome测序预测和验证靶基因。基因本体论（GO）富集分析表明，差异表达的miRNAs的靶基因因刺激应答、转录调控和离子转运功能而富集。由于差异表达的miRNAs的靶点多为与花发育相关的转录因子，如ARF、SPB（鳞状细胞启动子结合样蛋白），MADS-box（MCM1、AG、DEFA和SRF）、MYB、SPX（SYG 1、Pho 81和XPR 1）、TCP（TEOSINTE BRANCHED、Cycloidea和PCF）和PPR（五肽重复）基因，冷-结论小RNA转录组及其靶基因的分析为研究生殖器官发育过程中miRNA的调控提供了新的思路。在冷胁迫下发育小麦花序。miRNAs在冷胁迫反应中提供了另一层基因调控，可以通过遗传操作来减少小麦的产量损失。
BackgroundMicroRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in biotic and abiotic stresses by regulating their target genes. For common wheat, spring frost damage frequently occurs, especially when low temperature coincides with plants at early floral organ differentiation, which may result in significant yield loss. Up to date, the role of miRNAs in wheat response to frost stress is not well understood.ResultsWe report here the sequencing of small RNA transcriptomes from the young spikes that were treated with cold stress and the comparative analysis with those of the control. A total of 192 conserved miRNAs from 105 families and nine novel miRNAs were identified. Among them, 34 conserved and five novel miRNAs were differentially expressed between the cold-stressed samples and the controls. The expression patterns of 18 miRNAs were further validated by quantitative real time polymerase chain reaction (qRT-PCR). Moreover, nearly half of the miRNAs were cross inducible by biotic and abiotic stresses when compared with previously published work. Target genes were predicted and validated by degradome sequencing. Gene Ontology (GO) enrichment analysis showed that the target genes of differentially expressed miRNAs were enriched for response to the stimulus, regulation of transcription, and ion transport functions. Since many targets of differentially expressed miRNAs were transcription factors that are associated with floral development such as ARF, SPB (Squamosa Promoter Binding like protein), MADS-box (MCM1, AG, DEFA and SRF), MYB, SPX (SYG1, Pho81 and XPR1), TCP (TEOSINTE BRANCHED, Cycloidea and PCF), and PPR (PentatricoPeptide Repeat) genes, cold-altered miRNA expression may cause abnormal reproductive organ development.ConclusionAnalysis of small RNA transcriptomes and their target genes provide new insight into miRNA regulation in developing wheat inflorescences under cold stress. MiRNAs provide another layer of gene regulation in cold stress response that can be genetically manipulated to reduce yield loss in wheat.