Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver.
Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver.
复制标题
尿素合成的比较生物化学。
DOI：
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发表时间：
1959
影响因子：
4.8
通讯作者：
P. Cohen
中科院分区：
文献类型：
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作者：
G. W. Brown;P. Cohen
KrebsHenseleit鸟氨酸-尿素循环(1)的各种酶反应的阐明源于几个实验室(L-13)，其主要目的是分离、纯化和研究几种酶的要求和作用机理。本研究旨在为定量测定尿路结石动物肝脏中这些酶的水平提供一个系统的分析方案。首先，这些程序被用于研究从变态蝌蚪肝脏制备的无细胞提取物中这些酶的水平(18)。这些程序更广泛地用于比较研究这些酶在人体内的存在和水平。
Elucidation of the various enzymatic reactions of the KrebsHenseleit ornithine-urea cycle (1) stems from several laboratories (l-13), whose primary aims were the isolation, purification, and study of the requirements and mechanism of action of the several enzymes. The present study was undertaken to provide a systematic scheme of analysis for the quantitative determination of the levels of these enzymes in the livers of ureotelic animals. Primarily, the procedures were established to be used in connection with a study of levels of these enzymes in cell-free extracts prepared from the livers of metamorphosing tadpoles (18). The procedures have lent themselves more broadly to comparative studies on the occurrence and levels of these enzymes in mam-