Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals
Detection of JC virus DNA in peripheral blood cell subpopulations of HIV-1-infected individuals
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DOI：
10.3109/13550289909029484
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发表时间：
1999-08-01
影响因子：
3.2
通讯作者：
Letvin, NL
中科院分区：
文献类型：
--
作者：
Koralnik, IJ;Schmitz, JE;Letvin, NL
虽然有研究表明，JC病毒（JCV）在b淋巴细胞中从肾脏迁移到中枢神经系统，并在那里引发脱髓鞘，但这一阶段的JCV发病机制尚未得到系统的探讨。为了确定感染了JCV的外周血细胞亚群，用now细胞术纯化了hiv -1感染者和未感染对照组的单核细胞、粒细胞、T淋巴细胞和B淋巴细胞。所有这些细胞亚群均可通过PCR扩增检测到JCV DNA。这一发现表明，JCV在与白细胞的相互作用中缺乏特异性。
While it has been suggested that JC virus (JCV) migrates in B-lymphocytes from the kidney to the central nervous system where it initiates demyelination, this phase of JCV pathogenesis has not been systematically explored. To determine the peripheral blood cell subpopulation(s) infected with JCV, monocytes, granulocytes, and T and B lymphocytes from HIV-1-infected individuals and uninfected controls were purified by now cytometry. JCV DNA could be detected by PCR amplification in all of these cell subpopulations. This finding suggests that JCV lacks specificity in its interaction with leukocytes.