Adducin forms a bridge between the erythrocyte membrane and its cytoskeleton and regulates membrane cohesion
Adducin forms a bridge between the erythrocyte membrane and its cytoskeleton and regulates membrane cohesion
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DOI：
10.1182/blood-2009-02-203216
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发表时间：
2009-08-27
期刊：
影响因子：
20.3
通讯作者：
Low, Philip S.
中科院分区：
文献类型：
--
作者：
Anong, William A.;Franco, Taina;Low, Philip S.
红细胞膜骨架是最好理解的细胞骨架。因为它的蛋白质成分在几乎所有其他细胞中都有同源物，所以膜作为生物膜的基本模型。现代教科书将膜描绘为通过2个键连接到脂质双层的基于血影蛋白的2维膜骨架：带3-锚蛋白-β-血影蛋白和血型糖蛋白C-蛋白4.1-β-血影蛋白。(1-7)虽然有证据支持第一桥在调节膜内聚力中的重要作用，但血型糖蛋白C-蛋白4.1相互作用的破裂对膜稳定性几乎没有影响。(8)我们证明了存在一种新的带3加合蛋白血影蛋白桥，连接血影蛋白/肌动蛋白/蛋白4.1连接复合物的双层。由于这一桥的断裂导致自发的膜断裂，我们得出结论，带3-内收蛋白血影蛋白桥是重要的膜稳定性。所需的重新定位的部分带3人口的血影蛋白/肌动蛋白连接复合物和它的形成一个新的桥梁与内收蛋白需要一个显着的修订公认的模型的红细胞膜。（血。2009; 114：1904-1912）
The erythrocyte membrane skeleton is the best understood cytoskeleton. Because its protein components have homologs in virtually all other cells, the membrane serves as a fundamental model of biologic membranes. Modern textbooks portray the membrane as a 2-dimensional spectrin-based membrane skeleton attached to a lipid bilayer through 2 linkages: band 3-ankyrin-beta-spectrin and glycophorin C-protein 4.1-beta-spectrin.(1-7) Although evidence supports an essential role for the first bridge in regulating membrane cohesion, rupture of the glycophorin C-protein 4.1 interaction has little effect on membrane stability.(8) We demonstrate the existence of a novel band 3 adducin-spectrin bridge that connects the spectrin/actin/protein 4.1 junctional complex to the bilayer. As rupture of this bridge leads to spontaneous membrane fragmentation, we conclude that the band 3-adducin spectrin bridge is important to membrane stability. The required relocation of part of the band 3 population to the spectrin/actin junctional complex and its formation of a new bridge with adducin necessitates a significant revision of accepted models of the erythrocyte membrane. (Blood. 2009; 114: 1904-1912)