The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.
The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.
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DOI：
10.1083/jcb.127.3.737
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发表时间：
1994-11
期刊：
影响因子：
--
通讯作者：
Veenhuis M
中科院分区：
文献类型：
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作者：
Waterham HR;Titorenko VI;Haima P;Cregg JM;Harder W;Veenhuis M
本文报道了多态羊草（Hansenula polymorpha） PER1基因的克隆及其产物PER1p的鉴定。该基因是通过对H. polymorpha的per1突变体的功能互补克隆而来的，该突变体在过氧化物酶体基质蛋白（Pim-表型）的输入中受损。PER1的DNA序列预测PER1p是一个650个氨基酸的多肽，与其他已知蛋白质没有显著的序列相似性。PER1在野生型多态H.在葡萄糖上生长时表达量低但显著，在诱导过氧化物酶体增殖的任何一种底物上生长时表达量增加。PER1p含有羧基（PTS1）和氨基末端（PTS2）过氧化物酶体靶向信号，这两个信号都被证明能够将细菌β -内酰胺酶引导到细胞器。在野生型H. polymorpha中，PER1p是一种低丰度的蛋白质，被证明定位于过氧化物酶体基质中。我们的研究结果表明，PER1p进入过氧化物酶体是输入额外基质蛋白的先决条件，我们认为PER1p对过氧化物酶体蛋白质支持具有调节功能。
We describe the cloning of the Hansenula polymorpha PER1 gene and the characterization of the gene and its product, PER1p. The gene was cloned by functional complementation of a per1 mutant of H. polymorpha, which was impaired in the import of peroxisomal matrix proteins (Pim- phenotype). The DNA sequence of PER1 predicts that PER1p is a polypeptide of 650 amino acids with no significant sequence similarity to other known proteins. PER1 expression was low but significant in wild-type H. polymorpha growing on glucose and increased during growth on any one of a number of substrates which induce peroxisome proliferation. PER1p contains both a carboxy- (PTS1) and an amino- terminal (PTS2) peroxisomal targeting signal which both were demonstrated to be capable of directing bacterial beta-lactamase to the organelle. In wild-type H. polymorpha PER1p is a protein of low abundance which was demonstrated to be localized in the peroxisomal matrix. Our results suggest that the import of PER1p into peroxisomes is a prerequisite for the import of additional matrix proteins and we suggest a regulatory function of PER1p on peroxisomal protein support.