Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit
Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit
复制标题
激光治疗有助于在营养不足的情况下维持人类脱落乳牙（脱落）干细胞膜的完整性
DOI：
10.1007/s10103-018-2574-x
复制
发表时间：
2018
影响因子：
2.1
通讯作者：
V. T. Sakai
中科院分区：
文献类型：
--
作者：
Paula Corrêa Silveira da Silva;N. Marques;Marcella Tassi Farina;T. Oliveira;C. Duque;N. Marques;V. T. Sakai
本研究旨在分析激光照射对血清饥饿状态下人乳牙脱落干细胞膜完整性和活力的影响。营养缺陷被用来模拟再生牙科方法的SHED隔离的细胞应激条件，其中激光治疗可能是有益的。SHED在血清饥饿（MEMα + 1%FBS）下培养1或24小时（分别为方案A和B）。然后，细胞接受2.5 J/cm 2（0.10 W;组I和V）、5.0 J/cm 2（0.20 W;组II和VI）、7.5 J/cm 2（0.30 W;组III和VII）的低水平激光治疗（LLLT; 660 nm），或保持未照射（组IV和VIII）。在照射期间，将细胞维持在1%FBS（组I-IV）或10%FBS（正常培养条件;组V-VIII）中。通过定量乳酸脱氢酶（LDH）释放（照射后立即）评价膜完整性，并通过MTT测定（照射后24，48和72小时）评估细胞活力。血清饥饿并不改变LDH释放的非辐照SHED，而LDH释放显着减少组照射在1%的FBS（I和III），但不是在组照射在10%的FBS（V-VII），无论照射前的条件（方案A/B）。在大多数方案A组中，照射后24小时细胞活力显著较高。相比之下，方案B组中的细胞活力大多保持不变。LLLT有助于维持膜的完整性，在SHED受到营养不足之前和期间与0.10或0.30 W的照射。照射前短期血清饥饿改善照射后24小时的SHED活力。
This study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2(0.10 W; groups I and V), 5.0 J/cm2(0.20 W; groups II and VI), 7.5 J/cm2(0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation.