hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas
hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas
复制标题
DOI：
10.3892/mmr.2011.621
复制
发表时间：
2012-01-01
影响因子：
3.4
通讯作者：
Yang, Luoyan
中科院分区：
文献类型：
--
作者：
Wang, Yi;Luo, Hongmei;Yang, Luoyan
许多microRNA（miRNAs）在癌症发展中起着至关重要的作用。在这项研究中，我们报告说，hsa-miR-96在人膀胱尿路上皮癌中的表达水平高于正常组织。我们发现hsa-miR-96能增强人膀胱T24细胞的侵袭和分化能力，并促进其生长。hsa-miR-96的下调显着影响膀胱癌T24细胞的表型。与用空质粒载体或阴性对照miRNA抑制剂转染的细胞中的水平相比，用hsa-miR-96抑制剂转染的细胞中胰岛素受体底物1（IRS 1）和MAP 4K 1的mRNA和蛋白水平显著降低。总之，这些结果表明，hsa-miR-96可能通过上调IRSI和MAP 4K 1水平影响膀胱癌细胞的生长，在人膀胱尿路上皮癌中起着有希望的诊断标志物的作用。
Numerous microRNAs (miRNAs) play crucial roles in cancer development. In this study, we report that hsa-miR-96 is expressed at higher levels in human bladder urothelial carcinomas compared to normal tissues. We found that hsa-miR-96 increased invasion and differentiation of human bladder T24 cells and promoted their growth. Down-regulation of hsa-miR-96 significantly affected the phenotype of bladder cancer T24 cells. The mRNA and protein levels of insulin receptor substrate 1 (IRS1) and MAP4K1 were significantly reduced in cells transfected with the hsa-miR-96 inhibitor when compared with levels in cells transfected with the empty plasmid vector or the negative control miRNA inhibitor. Altogether, these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRSI and MAP4K1 levels, functioning as a promising diagnostic marker in human bladder urothelial carcinomas.