Identification and comparative analysis of accessory gland proteins in Orthoptera
Identification and comparative analysis of accessory gland proteins in Orthoptera
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DOI：
10.1139/g06-061
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发表时间：
2006-09-01
期刊：
影响因子：
3.1
通讯作者：
Swanson, Willie J.
中科院分区：
文献类型：
--
作者：
Braswell, W. Evan;Andres, Jose A.;Swanson, Willie J.
果蝇的附属生殖腺蛋白(Acp)进化迅速，在保证雄性受精成功方面起着重要作用。此外，ACP被认为参与了在近亲物种之间建立受精障碍的过程。虽然已知在许多昆虫类群的雄性中都存在附腺，但雄性在交配过程中传递给雌性的蛋白质在果蝇之外还没有得到很好的表征。为了更好地了解这些蛋白质，我们从两个蟋蟀物种的副腺中鉴定了EST，这两个物种是AlLonemobius Fasciatus和Gryllus ffciatus。使用表达序列标签(EST)方法，然后进行生物信息学和进化分析，我们发现许多蛋白质被分泌出来，因此，在交配过程中可以转移给雌性。此外，我们发现大多数EST都是新的，分类群之间的序列相似性很小。进化分析表明，板球蛋白受到多样化选择的影响，并表明AlLonemobius的多态程度比Gryllus低得多。尽管核苷酸序列迅速分化，但果蝇和蟋蟀类群之间似乎存在蛋白质类别的功能保守。
Accessory reproductive gland proteins (Acps) in Drosophila evolve quickly and appear to play an important role in ensuring the fertilization success of males. Moreover, Acps are thought to be involved in establishing barriers to fertilization between closely related species. While accessory glands are known to occur in the males of many insect groups, the proteins that are passed on to females by males during mating have not been well characterized outside of Drosophila. To gain a better understanding of these proteins, we characterized ESTs from the accessory glands of two cricket species, Allonemobius fasciatus and Gryllus firmus. Using an expressed sequence tag (EST) approach, followed by bioinformatic and evolutionary analyses, we found that many proteins are secreted and, therefore, available for transfer to the female during mating. Further, we found that most ESTs are novel, showing little sequence similarity between taxa. Evolutionary analyses suggest that cricket proteins are subject to diversifying selection and indicate that Allonemobius is much less polymorphic than Gryllus. Despite rapid nucleotide sequence divergence, there appears to be functional conservation of protein classes among Drosophila and cricket taxa.