Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources
Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources
复制标题
卡里蒙贾瓦群岛海参刺参的开发作为海洋生物资源的生产
DOI：
10.1088/1755-1315/116/1/012039
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
E. Yudiati
中科院分区：
文献类型：
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作者：
D. Pringgenies;S. Rudiyanti;E. Yudiati
本研究的目的是研究穿山甲中氨基酸、软骨素和葡萄糖胺含量的测定，发现其抗菌和抗癌作用。样品在分离前被冲洗，研究中只使用了样品体。采用高效液相色谱法对海参提取物进行氨基酸、软骨素和葡萄糖胺含量的测定。然后用扩散法对样品进行几种病原菌菌株的生物活性试验。采用人卵巢癌细胞株（KOC7C）法进行抗肿瘤实验。研究表明，石竹提取物具有抑制活性卵巢癌细胞生长的作用。定性实验表明，海参提取物对金黄色葡萄球菌、大肠杆菌、牛弧菌、假单胞菌等病原菌均有抑制作用。HPLC测定结果表明，海参提取物中氨基酸含量最高，为胶原蛋白（11200 mg/100g），其次为甘氨酸（3760 mg）、谷氨酸（3700 mg）、天冬氨酸（2540 mg）、丙氨酸（2140 mg）、脯氨酸（2050 mg）、精氨酸（2050 mg）、酪氨酸（1430 mg）、苏氨酸（1270 mg）。亮氨酸（1170）、缬氨酸（1050）、丝氨酸（971）、异亮氨酸（816）、苯丙氨酸（713）、赖氨酸（639）、蛋氨酸（383）、胱氨酸（263）和组氨酸（208）。提取物中还含有硫酸软骨素（4200）和盐酸氨基葡萄糖（<5.00）。综上所述，研究发现穿山甲提取物具有抗癌潜力，特别是对卵巢癌的抗癌作用，氨基酸中胶原蛋白含量最高，同时软骨素和葡萄糖胺含量也最高。
This research aim was to study the potential of Stichopus hermanii to determine the amino acid, chondroitin, and glucosamine contents, to discover its antibacterial and anti-cancer agent. The samples were rinsed prior to separation, with only the corpus being used in the study. Sea cucumber extract was then processed using HPLC to trace contents of amino acid, chondroitin, and glucosamine contents. The samples were then put into test against several strains of pathogenic bacteria by means of diffusion for any biological activity. The anti-cancer test was performed by human ovarian cancer cell line (KOC7C) method. The study showed that the extract of Stichopus hermanii has the potency to inhibit the growth of active ovarian cancer cells. The qualitative test of the sea cucumber extract showed that it is capable of suppressing the growth of several strains of pathogenic bacteria identified as Staphylococcus aureus, Escherichia coli, Vibrio voinivica, and Pseudomonas sp. HPLC results showed that the extract contained amino acid (mg/100g), the highest being Collagen (11200), followed by Glycine (3760), Glutamic Acid (3700), Aspartic Acid (2540), Alanine (2140), Proline (2050), Arginine (2050), Tyrosine (1430), Threonine (1270), Leucine (1170), Valine (1050), Serine (971), Isoleucine (816), Phenylalanine (713), Lysine (639), Methionine (383), Cystine (263) and Histidine (208). The extract also contained Chondroitin Sulfate (4200) and Glucosamine Hydrochloride (<5.00). In conclusion, the study found that the extract of Stichopus hermanii has potential as anti-cancer, particularly against ovarian cancer, with the highest content being Collagen within amino acids, as well as chondroitin and glucosamine.