Methane oxidation in a temperate coniferous forest soil: effects of inorganic N
Methane oxidation in a temperate coniferous forest soil: effects of inorganic N
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DOI：
10.1016/s0038-0717(02)00294-8
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发表时间：
2003-03
影响因子：
9.7
通讯作者：
Zhi-Ping Wang;P. Ineson
中科院分区：
文献类型：
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作者：
Zhi-Ping Wang;P. Ineson
甲烷氧化速率测定土壤中获得的针叶林在北方英格兰。在一系列实验室培养中研究了深度和添加的K+（K2 SO 4）、NH 4+（（NH 4）2SO 4）和NO3−（KNO 3）对潜在CH 4氧化的影响。腐殖土（H）层的CH 4氧化速率比其他土层大得多，当初始浓度为10和1000μl CH 4l −1时，最大速率分别为53和226 ng CH 4gdw −1h− 1。K+、NH 4+和NO3-的加入对CH 4的氧化表现出不同程度的抑制作用，其抑制作用随CH 4初始浓度、加入的离子和离子浓度的变化而变化。一般来说，在其他条件相同的情况下，与10μ l CH 4l − 1相比，在初始浓度为1000μ l CH 4l − 1的孵育液中，离子的抑制作用略大。K+和NH 4+处理的抑制率通常小于15%，但在高浓度K+和NH 4+处理时抑制率可达50%，在相同浓度下NH 4+的抑制效果始终略大于K+。与NH 4+形成鲜明对比的是，NO3−表现出非常强的抑制作用。在厌氧“微区”中通过添加NO3−还原产生的添加NO3−和NO2−可能对CH 4氧化细菌有毒。这些结果与其他报告的结果一起表明，NO3−在抑制森林土壤中CH 4氧化方面可能比NH 4+具有更大的重要性，需要在各种森林的广泛土壤类型中进行研究。
Methane oxidation rates were measured in soils obtained from a coniferous forest in northern England. The effects of depth and added K+(K2SO4), NH4+((NH4)2SO4) and NO3−(KNO3) on potential CH4oxidation were investigated in a series of laboratory incubations. The humus (H) layer soil showed much greater CH4oxidation rates than the other soil layers, with maximal rates of 53 and 226ng CH4gdw−1h−1when incubated with initial 10 and 1000μl CH4l−1, respectively. Additions of the solutes K+, NH4+and NO3−showed differing degrees of inhibition on CH4oxidation, which varied with the initial CH4concentration, the ion added, and the ion concentration. In general, inhibition by the ions was slightly greater for incubations with an initial concentration of 1000μl CH4l−1than for 10μl CH4l−1under otherwise identical conditions. For K+and NH4+treatments, inhibitory rates were usually less than 15%, but at high K+and NH4+concentrations inhibition could reach 50%, the inhibitory effects of NH4+were consistently slightly greater than those of K+at the same concentration. In marked contrast to NH4+, NO3−showed a very strong inhibitory effect. Added NO3−and NO2−produced via added NO3−reduction in anaerobic ‘microsites’ are probably toxic to CH4-oxidizing bacteria. These results, together with those from other reports, suggest that NO3−may have a greater importance in the inhibition of CH4oxidation in forest soils than that attributed to NH4+and needs to be investigated in a wide range of soil types from various forests.