Preparation and chiral recognition ability of crosslinked beads of polysaccharide derivatives.
Preparation and chiral recognition ability of crosslinked beads of polysaccharide derivatives.
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DOI：
10.1002/jssc.200600438
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发表时间：
2007-05
影响因子：
3.1
通讯作者：
Tomoyuki Ikail;C. Yamamoto;M. Kamigaito;Y. Okamoto
中科院分区：
文献类型：
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作者：
Tomoyuki Ikail;C. Yamamoto;M. Kamigaito;Y. Okamoto
制备了由带有部分羟基的纤维素3,5-二甲基苯基氨基甲酸酯组成的球形珠，用作HPLC的手性填料(CPM)。这些珠子是在不使用大孔硅胶的情况下获得的，大孔硅胶通常用作基于多糖衍生物的 CPM 的载体。珠子与二异氰酸酯如4,4'-二苯基甲烷二异氰酸酯(MDI)、4,4'-二苄基二异氰酸酯(DBDI)、甲苯-2,4-二异氰酸酯(TDI)和间苯二甲撑二异氰酸酯(XDI)交联后，将所得珠子填充到HPLC柱中。随着纤维素衍生物羟基含量的降低，所得CPM表现出更高的手性识别能力。与通过将纤维素衍生物涂覆在硅胶上制备的CPM相比，该珠具有更高的负载能力。交联珠可与含有氯仿的洗脱液一起使用。还制备了直链淀粉衍生物珠作为手性 HPLC 的 CPM。
The spherical beads consisting of cellulose 3,5-dimethylphenylcarbamate with partial hydroxyl groups were prepared to be used as chiral packing materials (CPMs) for HPLC. The beads were obtained without using macroporous silica gel, which is usually used as the support of the CPMs based on the polysaccharide derivatives. After the crosslinking in the bead with diisocyanates, such as 4,4'-diphenylmethane diisocyanate (MDI), 4,4'-dibenzyl diisocyanate (DBDI), tolylene-2,4-diisocyanate (TDI), and m-xylylene diisocyanate (XDI), the obtained beads were packed into an HPLC column. As the content of the hydroxyl groups of the cellulose derivatives decreased, the obtained CPM exhibited a higher chiral recognition ability. The beads possessed a higher loading capacity than the CPM prepared by coating the cellulose derivative on silica gel. The crosslinked beads could be used with the eluent containing chloroform. The amylose derivative beads were also prepared as a CPM for chiral HPLC.