Naturally occurring and induced neuronal death in the chick embryo in vivo requires protein and RNA synthesis: evidence for the role of cell death genes.
Naturally occurring and induced neuronal death in the chick embryo in vivo requires protein and RNA synthesis: evidence for the role of cell death genes.
复制标题
鸡胚体内自然发生和诱导的神经元死亡需要蛋白质和 RNA 合成：细胞死亡基因作用的证据。
DOI：
10.1016/0012-1606(90)90180-q
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发表时间：
1990
影响因子：
2.7
通讯作者：
Homma,S
中科院分区：
文献类型：
--
作者：
Oppenheim,RW;Prevette,D;Tytell,M;Homma,S
在运动神经元和背根神经节细胞的正常细胞死亡高峰期（胚胎第8天），用蛋白质和RNA合成抑制剂处理鸡胚卵10-12小时，可显著减少这些群体中退化神经元的数量。早期肢体缺失引起的大量神经元死亡也几乎完全被这些药物阻断。此外，在正常细胞死亡期结束时（胚胎第10天），外周轴突切断术后神经元的死亡通过生物合成反应抑制剂治疗显著减少。这些结果表明，在体内，自然发生的神经元死亡，神经元死亡诱导的外周靶点的情况下，和轴突切断诱导的神经元死亡后，在发展都需要积极的基因表达和蛋白质和RNA的合成。因此，各种情况下的神经元死亡可能反映了发育命运的表达，而发育命运通常只能被特定的环境信号（例如，神经营养分子）。
Treatment of chick embryosin ovofor 10–12 hr with inhibitors of protein and RNA synthesis during the peak time of normal cell death (Embryonic Day 8) for motoneurons and dorsal root ganglion cells markedly reduces the number of degenerating neurons in these populations. The massive neuronal death induced by the early absence of the limbs was also blocked almost completely by these agents. Further, the death of neurons following peripheral axotomy at the end of the normal cell death period (Embryonic Day 10) was reduced significantly by treatment with inhibitors of biosynthetic reactions. These results indicate that,in vivo, naturally occurring neuronal death, neuronal death induced by the absence of peripheral targets, and axotomy-induced neuronal death later in development all require active gene expression and protein and RNA synthesis. Therefore, neuronal death in a variety of situations may reflect the expression of a developmental fate that can normally only be overridden or suppressed by specific environmental signals (e.g., neurotrophic molecules).