Thy-1+ dendritic epidermal cells belong to the T-cell lineage.
Thy-1+ dendritic epidermal cells belong to the T-cell lineage.
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Thy-1 树突状表皮细胞属于 T 细胞谱系。
DOI：
10.1073/pnas.84.8.2430
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发表时间：
1987
影响因子：
11.1
通讯作者：
Shevach,EM
中科院分区：
文献类型：
--
作者：
Stingl,G;Gunter,KC;Tschachler,E;Yamada,H;Lechler,RI;Yokoyama,WM;Steiner,G;Germain,RN;Shevach,EM
鼠表皮含有树突状Thy-1+细胞群（Thy-1+ DEC）。虽然现在很清楚这些细胞是骨髓来源的，但广泛的形态学、组织化学和细胞膜标记物研究尚未明确将它们置于任何已知的造血分化途径中。基于Thy-1+ DEC可以在ConA和白细胞介素2的体外增殖的观察，我们建立了三个细胞系（Tehy 184，Tehy 245和Yety 245），它们可以在含有凝集素-淋巴因子-丰富的大鼠脾细胞培养上清液的培养基中连续生长。除了丧失与抗脱唾液酸-GM 1抗体（Tehy 184和Tehy 245）的反应性和获得白介素2受体表达外，培养的细胞系具有与新鲜分离的Thy-1+ DEC相同的表面特征：Thy-1+、Ly-5+、Lyt-1-、Lyt-2-、L3 T4-、Ia-、sIg-。使用Southern和RNA凝胶印迹分析，我们现在证明这些Thy-1+ DEC衍生的细胞系在编码T细胞受体（相关）β和γ链的基因复合物中表现出各种重排模式，并包含来自T细胞受体α和β链基因的成熟和/或不完整转录物，以及来自受体相关γ链基因的转录物。Tehy 184细胞是唯一同时含有成熟α链和β链转录本的细胞，与F23.1单克隆抗体反应呈阳性，F23.1单克隆抗体识别T细胞受体β链可变区基因片段的一个子集的产物。该抗体从这些细胞中沉淀84-88 kDa的表面蛋白，其在还原后分离成两条40- 44-kDa的链，这是Ti α/β异二聚体的特征。这些数据强烈表明，Thy-1+ DEC属于T细胞谱系，并指出表皮作为未成熟胸腺细胞迁移或胸腺外T细胞分化的位点。
The murine epidermis contains a population of dendritic, Thy-1+ cells (Thy-1+ DEC). Although it is now clear that these cells are of bone marrow origin, extensive morphological, histochemical, and cell-membrane marker studies have not definitively placed them in any known hematopoietic differentiation pathway. Based on the observation that Thy-1+ DEC can be propagated in vitro with Con A and interleukin 2, we have established three cell lines (Tehy 184, Tehy 245, and Yety 245) that can be continuously grown in medium with lectin-lymphokine-rich culture supernatants of rat spleen cells. With the exception of the loss of reactivity with anti-asialo-GM1 antibodies (Tehy 184 and Tehy 245) and the gain of interleukin 2 receptor expression, the cultured cell lines bear the same surface characteristics as freshly isolated Thy-1+ DEC: Thy-1+, Ly-5+, Lyt-1-, Lyt-2-, L3T4-, Ia-, sIg-. Using Southern and RNA gel blot analysis, we now demonstrate that these Thy-1+ DEC-derived cell lines exhibit various patterns of rearrangements in the gene complexes encoding the T-cell receptor (related) beta and gamma chains and contain mature and/or incomplete transcripts from the T-cell receptor alpha- and beta-chain genes, as well as transcripts from the receptor-related gamma-chain genes. Tehy 184 cells, the only cells containing both mature alpha- and beta-chain transcripts, react positively with the F23.1 monoclonal antibody, which recognizes the product of a subset of T-cell receptor beta-chain variable region gene segments. This antibody precipitates a surface protein of 84-88 kDa from these cells that after reduction separates into two 40- to 44-kDa chains, characteristic of Ti alpha/beta heterodimers. These data strongly suggest that Thy-1+ DEC belong to the T-cell lineage and point to the epidermis as a site either of immature thymocyte migration or of extrathymic T-cell differentiation.