Do rat hepatic microsomes contain multiple NADPH-supported fatty acid chain elongation pathways or a single pathway?
Do rat hepatic microsomes contain multiple NADPH-supported fatty acid chain elongation pathways or a single pathway?
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大鼠肝微粒体包含多个 NADPH 支持的脂肪酸链延长途径还是单一途径？
DOI：
10.1016/0006-291x(86)91059-4
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发表时间：
1986
影响因子：
3.1
通讯作者：
Cinti,DL
中科院分区：
文献类型：
--
作者：
Nagi,MN;Cook,L;Prasad,MR;Cinti,DL
化学合成了[14 C]-反式-2-十六碳酰辅酶A(16：1)和[2-14 C]-反式-2-顺式-8，11，14-二十碳五烯酰辅酶A(20：4)，并将其作为脂肪酸链延长系统中肝微体反式-2-烯基辅酶A还原酶组分的竞争底物。7.5μM和15μM 20：4竞争性地抑制16：1辅酶A向棕榈酰辅酶A的还原。此外，两种底物的还原都被乙炔衍生物DEC-2-ynoyl CoA以相同的程度抑制。此外，当底物为16：1、20：4和反式-2-顺-11-十八碳二烯基辅酶A(18：2)时，胰蛋白酶、糜蛋白酶和枯草杆菌酶抑制反式-2-烯基辅酶A还原酶的活性。这些结果与多个缩合酶连接到一个延伸途径的假说是一致的。
14 C]-trans-2-hexadecenoyl CoA (16: 1) and [2-14 C]-trans-2-cis-8, 11, 14-eicosatetraenoyl CoA (20: 4) were chemically synthesized and employed as competitive substrates for the liver microsomal trans-2-enoyl CoA reductase component of the fatty acid chain elongation system. Both 7.5 μM and 15 μM 20: 4 competitively inhibited the reduction of 16: 1 CoA to palmitoyl CoA. In addition, the reduction of both substrates was identically inhibited to the same extent by the acetylenic derivative, dec-2-ynoyl CoA. Furthermore, trypsin, chymotrypsin and subtilisin inhibited trans-2-enoyl CoA reductase activity when three different substrates were employed—16: 1, 20: 4 and trans-2-cis-11-octadecadienoyl CoA (18: 2). These results are consistent with the hypothesis of multiple condensing enzymes connected to a single elongation pathway.