Transient and stable transfection in the protozoan parasite Entamoeba invadens
Transient and stable transfection in the protozoan parasite Entamoeba invadens
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DOI：
10.1016/j.molbiopara.2012.04.007
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发表时间：
2012-07-01
影响因子：
1.5
通讯作者：
Singh, Upinder
中科院分区：
文献类型：
--
作者：
Ehrenkaufer, Gretchen M.;Singh, Upinder
溶组织内阿米巴是一种重要的人类病原体，也是世界范围内的主要健康问题。寄生虫生物学的许多方面都可以研究，但阶段转换除外，这在E.溶组织剂入侵内阿米巴在体外可高效地进行包囊和脱囊，是研究阶段转换的重要模型系统。然而，使用E.入侵者受到该物种缺乏遗传工具的限制。在这里，我们报告了一种新的方法，瞬时和稳定转染E。入侵。这些新工具将大大加强对内阿米巴发展的研究。(C)2012 Elsevier B.V.保留所有权利。
Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development. (C) 2012 Elsevier B.V. All rights reserved.