Morphological and electrophysiological properties of dissociated primate retinal cells
Morphological and electrophysiological properties of dissociated primate retinal cells
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DOI：
10.1016/s0006-8993(00)02614-7
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发表时间：
2000-09-01
期刊：
影响因子：
2.9
通讯作者：
Wu, SM
中科院分区：
文献类型：
--
作者：
Han, Y;Jacoby, RA;Wu, SM
虽然分离的视网膜细胞制备物已被广泛用于研究低等脊椎动物的视网膜功能，但来自灵长类动物视网膜的解离细胞尚未被开发用于常规生理学实验。在这项研究中，我们证明了从灵长类动物视网膜中获得可存活和可识别的游离细胞的可行性。此外，我们的特点是电压依赖性膜电流在每种类型的灵长类动物视网膜细胞与全细胞膜片钳技术。多种类型的离子电导与独特的电流分布记录在各种类型的灵长类动物视网膜神经元。光感受器表现出一个内向的I-H激活的膜超极化和外向电流激活的去极化电位。从双极细胞记录了两种类型的钾电流（瞬时钾电流I-K（A）和延迟整流钾电流I-K（V））。I-K（A）在侏儒双极细胞中占主导地位，而I-K（V）主要与视杆双极细胞有关。在有轴突终末的灵长类动物双极细胞中观察到L型钙电流（I-Ca），但在切断轴突的双极细胞中未观察到。大的电压依赖性钠电流（I-Na）只记录从神经节细胞。Muller细胞显示I-K（V）和大的钾内向整流电流（I-K（IR）），偶见小的I-Na。还研究了具有无长突细胞和水平细胞的电生理特征的神经元，即使它们的形态特征在细胞解离过程中丢失。通过使用本报告中概述的形态学和生理学标准，可以使用解离的视网膜细胞制备物作为灵长类动物视觉系统的生理学、生化学和药理学研究的体外系统。(C)2000 Elsevier Science B. V.保留所有权利。
Although isolated retinal cell preparations have been used widely to study retinal function in lower vertebrates, dissociated cells from primate retina have not been developed for routine physiological experiments. In this study, we demonstrated the feasibility of obtaining viable and identifiable dissociated cells from the primate retina. In addition, we characterized voltage-dependent membrane currents in each type of primate retinal cell with the whole-cell patch-clamp technique. Multiple types of ionic conductance with distinctive current profiles were recorded in various types of primate retinal neurons. Photoreceptors exhibited an inward I-H activated by membrane hyperpolarization and an outward current activated at depolarized potentials. Two types of potassium currents (transient potassium current, I-K(A), and delayed rectifier potassium current, I-K(V)) were recorded from bipolar cells. I-K(A) dominated the current response in putative midget bipolar cells, and I-K(V) was mainly associated with putative rod bipolar cells. L-type calcium currents (I-Ca) were observed in primate bipolar cells with axon terminals, but not in axotomized bipolar cells. Large voltage-dependent sodium currents (I-Na) were only recorded from ganglion cells. Muller cells exhibited I-K(V) and large potassium inward rectifier current (I-K(IR)), and occasionally a small I-Na. Neurons with electrophysiological signatures of amacrine cells and horizontal cells were also studied even though their morphological features were lost during cell dissociation. By using both morphological and physiological criteria outlined in this report, it is possible to use the dissociated retinal cell preparation as an in vitro system for physiological, biochemical and pharmacological studies of the primate visual system. (C) 2000 Elsevier Science B.V. All rights reserved.