Thermal and optical characterization of micro-LED probes for in vivo optogenetic neural stimulation
Thermal and optical characterization of micro-LED probes for in vivo optogenetic neural stimulation
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DOI：
10.1364/ol.38.000992
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发表时间：
2013-03-15
期刊：
影响因子：
3.6
通讯作者：
Mathieson, Keith
中科院分区：
文献类型：
--
作者：
McAlinden, Niall;Massoubre, David;Mathieson, Keith
在光遗传学领域，需要能够向大脑深层结构提供具有出色空间、时间和光谱分辨率的光的紧凑光源。在这里，我们展示了用于此类应用的定制 GaN 基 LED 探针，并分析了电学、光学和热性能。研究发现输出功率密度和发射光谱适合刺激视紫红质通道蛋白-2（channelrhodopsin-2），这是目前光遗传学中最常见的光敏蛋白之一。 LED 装置产生高光强度，远远超过刺激神经元内光敏蛋白所需的强度。还对热性能进行了研究，表明可以在脉冲模式下实现广泛的操作状态，同时保持大脑温度升高最小（0.5 摄氏度）。这种类型的定制设备代表了光遗传学界向前迈出的重要一步，允许沿着微创神经探针的长度存在多个明亮的激发位点。 (C) 2013 美国光学学会
Within optogenetics there is a need for compact light sources that are capable of delivering light with excellent spatial, temporal, and spectral resolution to deep brain structures. Here, we demonstrate a custom GaN-based LED probe for such applications and the electrical, optical, and thermal properties are analyzed. The output power density and emission spectrum were found to be suitable for stimulating channelrhodopsin-2, one of the most common light-sensitive proteins currently used in optogenetics. The LED device produced high light intensities, far in excess of those required to stimulate the light-sensitive proteins within the neurons. Thermal performance was also investigated, illustrating that a broad range of operating regimes in pulsed mode are accessible while keeping a minimum increase in temperature for the brain (0.5 degrees C). This type of custom device represents a significant step forward for the optogenetics community, allowing multiple bright excitation sites along the length of a minimally invasive neural probe. (C) 2013 Optical Society of America