Production, characterization, and functional analysis of newly established CD99 monoclonal antibodies MT99/1 and MT99/2
Production, characterization, and functional analysis of newly established CD99 monoclonal antibodies MT99/1 and MT99/2
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DOI：
10.1089/hyb.2007.0504
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发表时间：
2007-08-01
期刊：
影响因子：
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通讯作者：
Kasinrerk, Watchara
中科院分区：
文献类型：
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作者：
Khunkaewla, Panida;Chiampanichayakul, Sawitree;Kasinrerk, Watchara
白细胞表面分子CD99是由E2/MIC2基因编码的一种完整的膜糖蛋白。该分子在造血系统细胞中广泛表达，并呈现两种表面形式，长32 kDa形式和短28 kDa形式。虽然CD99分子的完整功能尚不清楚，但已有报道称其参与细胞粘附、迁移和凋亡的调节。因此，一些CD99单克隆抗体（mab）已经产生，用于CD99分子的生化和功能研究。本研究制备了两种CD99单克隆抗体MT99/1和MT99/2。单克隆抗体识别CD99分子的不同表位。单克隆抗体MT99/1适合生化鉴定，MT99/2不适合。MAb MT99/1与其表位结合可诱导细胞粘附和凋亡。生成的单克隆抗体可用于进一步研究CD99分子的功能和机制，包括其在免疫系统中的作用，并可能在肿瘤的诊断和治疗中具有应用价值。
The leukocyte surface molecule CD99 is an integral membrane glycoprotein encoded by the E2/MIC2 gene. This molecule is broadly expressed on cells of the hematopoietic system and displays two surface forms, a long 32 kDa form and a short 28 kDa form. While the complete function of the CD99 molecule is unclear, it has been reported to be involved in regulation of cell adhesion, migration, and apoptosis. Thus, several CD99 monoclonal antibodies (MAbs) have been generated for biochemical and functional studies of the CD99 molecule. In the present study two CD99 MAbs, MT99/1 and MT99/2, were produced. The MAbs recognized different epitopes of the CD99 molecule. MAb MT99/1, but not MT99/2, was appropriate for biochemical characterization. Binding of MAb MT99/1 with its epitope led to the induction of cell adhesion and apoptosis. The generated MAbs can be used for future study of the function and mechanism of the CD99 molecule, including its role in the immune system, and may have application in tumor diagnosis and treatment.