Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.

Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.
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DOI:
10.1007/978-1-0716-3139-3_13
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发表时间:
2023
期刊:
Methods in molecular biology (Clifton, N.J.)
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向新生和幼年小鼠递送药物和其他化合物,这些药物和化合物操纵生理学或细胞/分子状态-例如,通过激活或抑制信号通路)是研究精子发生的一种强大但未充分利用的方法。在这里,我们提供了我们在实验室中优化的安全有效地喂养和注射小鼠的详细方案,并讨论了故障排除方法。
The delivery, to newborn and juvenile mice, of drugs and other compounds that manipulate the physiology or cellular/molecular state -e.g., by activating or inhibiting signaling pathways) is a powerful, yet under-utilized approach to studying spermatogenesis. Here, we provide detailed protocols we have optimized in our laboratory for safely and effectively feeding and injecting mice and discuss troubleshooting approaches.