Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth
Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth
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大肠杆菌色氨酸转移核糖核酸连接酶的突变导致生长温度敏感性
DOI：
10.1007/bf00331002
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
L. Isaksson
中科院分区：
文献类型：
--
作者：
K. Bohman;L. Isaksson
具有色氨酰-tRNA连接酶改变的大肠杆菌K-12菌株对生长具有温度敏感性，已被分离为自发一步突变株。即使在允许的温度下，突变的酶的活性也与野生型显著不同。当在不允许的温度下进行检测时，突变酶完全失活。在其中一株突变菌株中，添加L-苯丙氨酸或L-酪氨酸可完全抑制其生长。
Strains ofEscherichia coliK-12 with altered tryptophanyl-tRNA ligases, conferring temperature-sensitivity for growth, have been isolated as spontaneous one-step mutants. The mutated enzymes differ markedly in activity from the wildtype, even at the permissive temperature. When assayed at the non-permissive temperature, the mutant enzymes are completely inactive. In one of the mutant strains, growth can be completely inhibited by addition of L-phenylalanine or L-tyrosine to the medium.