Inter-α-trypsin inhibitor heavy chain 4 is a novel marker of acute ischemic stroke
Inter-α-trypsin inhibitor heavy chain 4 is a novel marker of acute ischemic stroke
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DOI：
10.1016/j.cca.2009.01.009
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发表时间：
2009-04-01
影响因子：
5
通讯作者：
Daginawala, Hatim F.
中科院分区：
文献类型：
--
作者：
Kashyap, Rajpal S.;Nayak, Amit R.;Daginawala, Hatim F.
背景：炎症和炎症标志物在急性缺血性卒中（AIS）中起重要作用。在早期的研究中，我们发现了一种120 kDa的蛋白质，该蛋白质在健康参与者中高度表达，但在AIS患者中不表达或几乎不表达。本工作的目的是分离，表征和评估AIS患者血液样本中的120 kDa蛋白质。此外，我们还将该蛋白与S-100 β β、神经元特异性烯醇化酶（NSE）、细胞因子白细胞介素IL-2和IL-10进行了比较。然后，120-kDa的蛋白质进行了评估，使用酶联免疫吸附试验从AIS患者的血清样本，这是收集0，24，48，72和144 h后admission.Results：一个内部片段的氨基酸序列确定的120-kDa的蛋白质间α-胰蛋白酶抑制剂重链4（ITIH 4）。与对照组相比，AIS患者中ITIH 4蛋白完全缺失，并且随着病情的改善，AIS患者的血清水平恢复正常。S-100 beta的表达。结论：ITIH 4是一种抗炎蛋白，其在AIS的诊断和预后中的潜在应用值得进一步研究。(c)2009爱思唯尔有限公司版权所有。
Background: Inflammation and inflammatory markers play an important role in acute ischemic stroke (AIS). In an earlier study, we discovered a 120-kDa protein that was highly expressed in healthy participants, but either was not expressed or was barely expressed in AIS patients. The purpose of the present work was to isolate, characterize, and evaluate this 120-kDa protein in blood samples of AIS patients. In addition, we investigated how the identified protein compared with protein S-100 beta beta, neuron-specific enolase (NSE), and cytokine interleukins IL-2 and IL-10.Methods: The 120-kDa protein band was analyzed via LC-MS/MS analysis. Then, the 120-kDa protein was evaluated using an enzyme-linked immunosorbant assay in serum samples from AIS patients, which were collected 0, 24, 48, 72 and 144 h after admission.Results: The amino acid sequence of an internal fragment identified the 120-kDa protein as inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4). The ITIH4 protein was completely absent in AIS patients as compared to those in the control group, and serum levels returned to normal in AIS patients as their condition improved. Expression of S-100 beta beta. NSE, IL-2, and IL-10 were highly correlated with ITIH4 expression.Conclusions: ITIH4 is an anti-inflammatory protein, and suggests that further investigation into its potential use in the diagnosis and prognosis of AIS is war-ranted. (c) 2009 Elsevier B.V. All rights reserved.