FINE-STRUCTURE OF HUMAN MALARIA INVITRO
FINE-STRUCTURE OF HUMAN MALARIA INVITRO
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DOI：
10.1111/j.1550-7408.1978.tb04167.x
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
TRAGER, W
中科院分区：
文献类型：
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作者：
LANGRETH, SG;JENSEN, JB;TRAGER, W
本文用电镜观察了人恶性疟原虫的红细胞周期。在人类红细胞中连续培养的三种寄生虫株与Aotus猴体内感染进行了比较。体外连续培养不改变恶性疟原虫的超微结构。线粒体在红细胞生命周期的各个阶段都含有DNA样细丝和一些嵴。高尔基体在鞭毛体阶段是突出的，并且可能参与棒状体的形成。在培养中，首先在滋养体感染的红细胞表面出现球形突起。在体外细胞上出现结节的时间与从体内外周循环隔离的寄生虫的生命周期阶段相关。较老的寄生虫的突起物质从红细胞表面结合并形成延伸。有些物质从宿主细胞表面脱落。无论在体外还是体内，含成熟裂殖子的红细胞中的寄生虫空泡膜都会发生破裂。裂殖子的结构与诺氏裂殖子相似。培养中的未成熟配子母细胞没有结。
The erythrocytic cycle of the human malaria parasite, Plasmodium falciparum, was examined by EM copy. Three strains of parasites maintained in continuous culture in human erythrocytes were compared with in vivo infections in Aotus monkeys. The ultrastructure of P. falciparum is not altered by continuous cultivation in vitro. Mitochondria contain DNA-like filaments and some cristae at all stages of the erythrocytic life cycle. The Golgi apparatus is prominent at the schizont stage and may be involved in the formation of rhoptries. In culture, knob-like protrusions 1st appear on the surface of trophozoite-infected erythrocytes. The time of appearance of knobs on cells in vitro correlates with the life cycle stage of parasites which are sequestered from the peripheral circulation in vivo. Knob material of older parasites coalesces and forms extensions from the crythrocyte surface. Some of this material is sloughed from the host cell surface. The parasitophorous vacuole membrane breaks down in erythrocytes containing mature merozoites both in vitro and in vivo. Merozoite structure is similar to that of P. knowlesi. The immature gametocytes in culture have no knobs.