Detailed biosynthetic pathway to decaprenoxanthin diglucoside in Corynebacterium glutamicum and identification of novel intermediates
Detailed biosynthetic pathway to decaprenoxanthin diglucoside in Corynebacterium glutamicum and identification of novel intermediates
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DOI：
10.1007/s002030100315
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Sandmann, G
中科院分区：
文献类型：
--
作者：
Krubasik, P;Takaichi, S;Sandmann, G
分析了谷氨酸棒杆菌的类胡萝卜素突变体的类胡萝卜素含量。突变体MV10积累了与野生型相同的类胡萝卜素、十癸烯黄素、十癸烯黄素单葡萄糖苷和(2R,6R,2'R,6'R)-十癸烯黄素二-(β-D)-葡萄糖苷，但含量高出三倍。此外，十癸烯黄素二葡萄糖苷脂肪酸酯和中间体九烯丙二烯、2-(3-甲基-2-丁烯基)-ε-胡萝卜素和八叠碳烯、2,2'-双(3-甲基-2-丁烯基)-ε-ε-胡萝卜素被鉴定为次要类胡萝卜素。粉色突变体MV40和MV60仅合成番茄红素。从另一个粉红色突变体 MV70 中分离出新型 C-50-类胡萝卜素。通过NMR和质谱分析，九黄黄素，2-(4-羟基-3-甲基-2-丁烯基)-1,16-二脱氢-1,2-二氢-psi,psi-胡萝卜素和黄黄素，2,2'-双(4-羟基-3-甲基-2-丁烯基)-1,16,1',16'-四氢-1,2,1' ,2'-四氢-psi,psi-胡萝卜素被鉴定。这些中间体的鉴定揭示了谷氨酸棒杆菌中十癸烯黄素衍生物形成的详细途径。
Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2 'R,6 'R)-decaprenoxanthin di-(beta -D)-glucoside, but in three-fold higher amounts. In addition, decaprenoxanthin diglucoside fatty acid esters and the intermediates nonaprene, 2-(3-methyl-2-butenyl)-epsilon,psi -carotene, and sarcinene, 2,2 ' -bis(3-methyl-2-butenyl)-epsilon,epsilon -carotene were identified as minor carotenoids. The pink mutants MV40 and MV60 synthesized only lycopene. From another pink mutant, MV70, novel C-50-carotenoids were isolated. By NMR and mass spectroscopy, nonaflavuxanthin, 2-(4-hydroxy-3-methyl-2-butenyl)-1,16-didehydro-1,2-dihydro-psi,psi -carotene, and flavuxanthin, 2,2 ' -bis(4-hydroxy-3-methyl-2-butenyl)-1,16,1 ' ,16 ' -tetradehydro-1,2,1 ' ,2 ' -tetrahydro-psi,psi -carotene, were identified. The identification of these intermediates revealed the detailed pathway for the formation of decaprenoxanthin derivatives in Corynebacterium glutamicum.