Influence of Eimeria falciformis Infection on Gut Microbiota and Metabolic Pathways in Mice
Influence of Eimeria falciformis Infection on Gut Microbiota and Metabolic Pathways in Mice
复制标题
镰状艾美耳球虫感染对小鼠肠道微生物群和代谢途径的影响。
DOI：
10.1128/iai.00073-18
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发表时间：
2018-05-01
影响因子：
3.1
通讯作者：
Liu, Xianyong
中科院分区：
文献类型：
--
作者：
Huang, Guangping;Zhang, Sixin;Liu, Xianyong
球虫病是由不同种类的艾美耳球虫寄生虫引起的一种世界性的重要畜禽疾病。在这里，我们报告了以前未知的变化，在肠道微生物和代谢的BALB/c小鼠感染镰状艾美耳球虫。具体来说，我们观察到盲肠细菌丰度的显著变化，并破坏了寄生动物的代谢。毛螺菌科细菌NK 4A 136、瘤胃梭菌、Alistipes和乳杆菌的相对丰度随着E.镰状菌感染，而大肠杆菌、志贺菌、螺杆菌、克雷伯菌和拟杆菌增加。感染小鼠血清样品中的碳水化合物和氨基酸代谢物与未感染对照组相比发生了显著改变。包括天冬酰胺、组氨酸、L-半胱氨酸、色氨酸、赖氨酸、甘氨酸、丝氨酸、丙氨酸、脯氨酸、鸟氨酸、蛋氨酸和缬氨酸在内的氨基酸水平在感染后第7天下降，然后在第14天恢复到基线水平。此外，吲哚乳酸和甘露醇水平的增加以及草酸量的减少表明寄生虫感染后碳代谢受损。这些数据表明，肠道球虫感染扰乱微生物群，破坏碳和氮代谢。
Coccidiosis, caused by different species of Eimeria parasites, is an economically important disease of poultry and livestock worldwide. Here we report previously unknown alterations in the gut microbes and metabolism of BALB/c mice infected with Eimeria falciformis. Specifically, we observed a significant shift in the abundance of cecal bacteria and disrupted metabolism in parasitized animals. The relative abundances of Lachnospiraceae bacterium NK4A136, Ruminiclostridium, Alistipes, and Lactobacillus declined in response to E. falciformis infection, whereas Escherichia, Shigella, Helicobacter, Klebsiella, and Bacteroides were increased. Carbohydrate and amino acid metabolites in the serum samples of infected mice were significantly altered compared to naive controls. Levels of amino acids, including asparagine, histidine, L-cysteine, tryptophan, lysine, glycine, serine, alanine, proline, ornithine, methionine, and valine, decreased on day 7 postinfection before returning to baseline on day 14. In addition, increased levels of indolelactate and mannitol and a reduced amount of oxalic acid indicated impaired carbon metabolism upon parasitic infection. These data demonstrate that intestinal coccidial infection perturbs the microbiota and disrupts carbon and nitrogen metabolism.