ANTIPROLIFERATIVE EFFECT OF THE GARLIC COMPOUND S-ALLYL CYSTEINE ON HUMAN NEUROBLASTOMA-CELLS INVITRO
ANTIPROLIFERATIVE EFFECT OF THE GARLIC COMPOUND S-ALLYL CYSTEINE ON HUMAN NEUROBLASTOMA-CELLS INVITRO
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DOI：
10.1016/0304-3835(92)90263-u
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发表时间：
1992-04-30
期刊：
影响因子：
9.7
通讯作者：
SIDELL, N
中科院分区：
文献类型：
--
作者：
WELCH, C;WUARIN, L;SIDELL, N
从大蒜中提取的多种化合物已在动物系统中显示出具有抗癌特性。然而，关于大蒜在预防或治疗人类癌症方面的有效性的信息很少。在目前的研究中，我们评估了s -丙烯基半胱氨酸（SAC），一种老化大蒜提取物的衍生物，在体外影响LA-N-5人神经母细胞瘤细胞的增殖和分化的能力。在SAC处理至少2天的培养物中，观察到细胞生长抑制的时间和剂量依赖性，在大约600-mu-g/ml时，有一半的最大反应。通过形态学、生化和分子标记来评估，SAC治疗不能诱导神经母细胞瘤细胞分化。此外，SAC不能增强维甲酸和8-溴环AMP的作用，这两种已知的促进LA-N-5细胞分化的药物。我们的结果表明，SAC在体外可以抑制人神经母细胞瘤细胞的生长。然而，这种化合物明显不能诱导分化，这可能限制了它的治疗潜力。
A variety of compounds derived from garlic bulbs have been shown in animal systems to possess anticancer properties. However, little information is available regarding the effectiveness of garlic in the prevention or treatment of human cancers. In the current study, we have assessed the ability of S-allyl cysteine (SAC), a derivative of aged garlic extract, to affect the proliferation and differentiation of LA-N-5 human neuroblastoma cells in vitro. Time-and dose-dependent inhibition of cell grow was observed in cultures treated with SAC for at least 2 days, with a half-maximal response at approximately 600-mu-g/ml. SAC treatment was unable to induce differentiation in neuroblastoma cells as assessed by morphological, biochemical and molecular markers. In addition, SAC was unable to potentiate the effects of retinoic acid and 8-bromo-cyclic AMP, agents known to promote differentiation of LA-N-5 cells. Our results indicate that SAC can inhibit human neuroblastoma cell growth in vitro. However, the apparent inability of this compound to induce differentiation may limit its therapeutic potential.