Small auxin upregulated RNA (SAUR) gene family in maize: Identification, evolution, and its phylogenetic comparison with Arabidopsis, rice, and sorghum
Small auxin upregulated RNA (SAUR) gene family in maize: Identification, evolution, and its phylogenetic comparison with Arabidopsis, rice, and sorghum
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DOI：
10.1111/jipb.12127
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发表时间：
2014-02-01
影响因子：
11.4
通讯作者：
Cao, Jun
中科院分区：
文献类型：
--
作者：
Chen, Yuzhu;Hao, Xi;Cao, Jun
小生长素上调RNA（SAURs）是植物中早期生长素应答基因，代表了一个大的多基因家族。在此，我们从玉米（Zea mays subsp. Mays）通过重复的数据库搜索和人工注释。系统进化分析表明，拟南芥、水稻、高粱和玉米的SAUR蛋白可分为16个类群。这些基因在玉米染色体上呈非随机分布，片段重复和串联重复有助于玉米SAUR基因家族的扩展。同线性分析建立了玉米和高粱基因组中SAUR基因之间的同源关系和功能连锁。我们还发现，生长素反应元件在玉米SAUR成员的上游序列中是保守的。选择分析确定了一些显着的网站特定的限制作用于大多数SAUR旁系。基于微阵列数据的表达谱提供了对SAUR基因家族成员之间可能的功能分歧的见解。实时荧光定量PCR分析表明，随机选择的10个ZmSAUR基因中的一些至少可以在玉米的茎或根组织中被诱导。这些结果揭示了玉米SAUR基因家族的全面概述，并可能为破译其在植物发育过程中的功能铺平道路。
Small auxin-up RNAs (SAURs) are the early auxin-responsive genes represented by a large multigene family in plants. Here, we identified 79 SAUR gene family members from maize (Zea mays subsp. mays) by a reiterative database search and manual annotation. Phylogenetic analysis indicated that the SAUR proteins from Arabidopsis, rice, sorghum, and maize had divided into 16 groups. These genes were non-randomly distributed across the maize chromosomes, and segmental duplication and tandem duplication contributed to the expansion of the maize SAUR gene family. Synteny analysis established orthology relationships and functional linkages between SAUR genes in maize and sorghum genomes. We also found that the auxin-responsive elements were conserved in the upstream sequences of maize SAUR members. Selection analyses identified some significant site-specific constraints acted on most SAUR paralogs. Expression profiles based on microarray data have provided insights into the possible functional divergence among members of the SAUR gene family. Quantitative real-time PCR analysis indicated that some of the 10 randomly selected ZmSAUR genes could be induced at least in maize shoot or root tissue tested. The results reveal a comprehensive overview of the maize SAUR gene family and may pave the way for deciphering their function during plant development.