Immunoreactive corticotropin-releasing factor in rat plasma.
Immunoreactive corticotropin-releasing factor in rat plasma.
复制标题
大鼠血浆中的免疫反应性促肾上腺皮质激素释放因子。
DOI：
10.1210/endo-120-4-1391
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发表时间：
1987
期刊：
影响因子：
4.8
通讯作者：
K. Shizume
中科院分区：
文献类型：
--
作者：
T. Sumitomo;T. Suda;N. Tomori;F. Yajima;Y. Nakagami;T. Ushiyama;H. Demura;K. Shizume
在肾上腺切除术后和胰岛素诱导的低血糖中测定大鼠垂体前叶和血浆中的免疫反应性促肾上腺皮质激素（I-ACTH）水平，以及正中隆起（ME）和血浆中的免疫反应性促肾上腺皮质激素释放因子（I-CRF）浓度。在正常大鼠的血浆中检测到 I-CRF（平均值 +/- SD，5.6 +/- 0.9 pg/ml；n = 6）。来自这些大鼠的混合血浆的 I-CRF 的凝胶过滤色谱显示在真实大鼠 CRF 的位置洗脱的单个峰。 ME中的I-CRF水平和垂体前叶中的I-ACTH水平在肾上腺切除术后立即下降，然后在术后14天逐渐升高至高水平。手术后血浆I-CRF和I-ACTH浓度立即升高，24小时略微下降至接近对照水平，然后在术后14天升高至高浓度。肾上腺切除术后 14 天，随后每日补充地塞米松，血浆和 ME I-CRF 水平几乎与对照水平相同。在胰岛素引起的低血糖中，血浆 I-ACTH 和 I-CRF 浓度在 30 分钟和 60 分钟时升高，而 ME I-CRF 含量降低。这些结果表明血浆 I-CRF 水平反映了下丘脑 CRF 水平的变化。
Immunoreactive ACTH (I-ACTH) levels in the rat anterior pituitary and plasma, and immunoreactive corticotropin-releasing factor (I-CRF) concentrations in the median eminence (ME) and plasma were determined after adrenalectomy and in insulin-induced hypoglycemia. I-CRF was detected in plasma from normal rats (mean +/- SD, 5.6 +/- 0.9 pg/ml; n = 6). Gel filtration chromatography of I-CRF from pooled plasma of these rats revealed a single peak which eluted in the position of authentic rat CRF. I-CRF levels in ME and I-ACTH levels in anterior pituitary decreased immediately after adrenalectomy, then gradually increased to high levels 14 days after surgery. Plasma I-CRF and I-ACTH concentrations increased immediately after surgery, slightly decreased to near the control levels at 24 h, and then increased to high concentrations 14 days after surgery. Plasma and ME I-CRF levels 14 days after adrenalectomy, followed by daily dexamethasone replacement, were almost the same as control levels. In insulin-induced hypoglycemia, plasma I-ACTH and I-CRF concentrations increased and ME I-CRF content decreased at 30 and 60 min. These results suggest that plasma I-CRF levels reflect changes in hypothalamic CRF levels.