Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.
Circulating mRNAs and miRNAs as candidate markers for the diagnosis and prognosis of prostate cancer.
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DOI：
10.1371/journal.pone.0184094
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Cólus IMS
中科院分区：
文献类型：
--
作者：
Souza MF;Kuasne H;Barros-Filho MC;Cilião HL;Marchi FA;Fuganti PE;Paschoal AR;Rogatto SR;Cólus IMS
循环核酸以游离形式存在于体液中，可作为癌症诊断和预后的微创工具。只有少数研究调查了循环mrna和microRNAs （miRNAs）在前列腺癌（PCa）中的潜在应用。使用癌症基因组图谱（TCGA）数据库进行计算机分析，以确定循环mRNA和miRNA作为PCa的潜在标记物。在正常和肿瘤样本中，共有2267个基因和49个mirna存在差异表达。靶基因预测分析及mRNA和miRNA表达综合分析显示，102例未经治疗的PCa患者和50例无癌个体的血浆样本中有11个基因和8个miRNA被RT-qPCR验证。OR51E2和SIM2两个基因以及miR-200c和miR-200b两个mirna与PCa有显著相关性。这些转录物的表达水平将PCa患者与对照组区分开来（67%的敏感性和75%的特异性）。根据前列腺特异性抗原(PSA)≤4.0 ng/mL的前列腺癌患者和对照组的OR51E2和SIM2表达水平进行区分。miR-200c表达与Gleason评分、miR-200b、骨转移、双侧肿瘤、PSA bb0 10.0 ng/mL相关。循环mRNA和miRNA的联合检测有助于前列腺癌的诊断和预后。
Circulating nucleic acids are found in free form in body fluids and may serve as minimally invasive tools for cancer diagnosis and prognosis. Only a few studies have investigated the potential application of circulating mRNAs and microRNAs (miRNAs) in prostate cancer (PCa). The Cancer Genome Atlas (TCGA) database was used for an in silico analysis to identify circulating mRNA and miRNA as potential markers of PCa. A total of 2,267 genes and 49 miRNAs were differentially expressed between normal and tumor samples. The prediction analyses of target genes and integrative analysis of mRNA and miRNA expression revealed eleven genes and eight miRNAs which were validated by RT-qPCR in plasma samples from 102 untreated PCa patients and 50 cancer-free individuals. Two genes, OR51E2 and SIM2, and two miRNAs, miR-200c and miR-200b, showed significant association with PCa. Expression levels of these transcripts distinguished PCa patients from controls (67% sensitivity and 75% specificity). PCa patients and controls with prostate-specific antigen (PSA) ≤ 4.0 ng/mL were discriminated based on OR51E2 and SIM2 expression levels. The miR-200c expression showed association with Gleason score and miR-200b, with bone metastasis, bilateral tumor, and PSA > 10.0 ng/mL. The combination of circulating mRNA and miRNA was useful for the diagnosis and prognosis of PCa.
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将转录因子识别为单一的同源物2作为前列腺癌中潜在的生物标志物和免疫疗法靶标。
DOI：
10.1158/1078-0432.ccr-09-0911
发表时间：
2009-09-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Arredouani MS;Lu B;Bhasin M;Eljanne M;Yue W;Mosquera JM;Bubley GJ;Li V;Rubin MA;Libermann TA;Sanda MG
通讯作者：
Sanda MG
影响因子：
--
作者：
Agaoglu, Fulya Yaman;Kovancilar, Muge;Gezer, Ugur
通讯作者：
Gezer, Ugur
影响因子：
6.4
作者：
Brase, Jan C.;Johannes, Marc;Sueltmann, Holger
通讯作者：
Sueltmann, Holger
影响因子：
3.8
作者：
Erdmann K;Kaulke K;Thomae C;Huebner D;Sergon M;Froehner M;Wirth MP;Fuessel S
通讯作者：
Fuessel S
影响因子：
3.7
作者：
Duttagupta R;Jiang R;Gollub J;Getts RC;Jones KW
通讯作者：
Jones KW