Two Techniques for Simultaneous Identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by Use of the Small-Subunit rRNA Gene
Two Techniques for Simultaneous Identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by Use of the Small-Subunit rRNA Gene
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DOI：
10.1128/jcm.02180-12
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发表时间：
2012-12-01
影响因子：
9.4
通讯作者：
Noedl, Harald
中科院分区：
文献类型：
--
作者：
Fuehrer, Hans-Peter;Stadler, Marie-Therese;Noedl, Harald
传统上用于检测卵形疟原虫感染的引物单独使用时，已知不能结合小亚单位rRNA基因内的所有卵形疟原虫。我们描述了一种简单、经济、省时的多重套式聚合酶链式反应和套式聚合酶链式反应，该套式聚合酶链式反应和套式聚合酶链式反应使用了一组新的引物来同时检测卵形芽胞杆菌和卵形芽胞杆菌。
The primers traditionally used to detect Plasmodium ovale infections are known for not binding all P. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection of P. ovale curtisi and P. ovale wallikeri.