A strain isolated from gas oil-contaminated soil displays chemotaxis towards gas oil and hexadecane
A strain isolated from gas oil-contaminated soil displays chemotaxis towards gas oil and hexadecane
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DOI：
10.1046/j.1462-2920.2003.00507.x
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发表时间：
2003-10-01
影响因子：
5.1
通讯作者：
Studdert, CA
中科院分区：
文献类型：
--
作者：
Lanfranconi, MP;Alvarex, HM;Studdert, CA
在这份报告中，我们描述了分离的菌株从土壤中污染的瓦斯油采取细菌从一个趋化环瓦斯油含有软琼脂平板。16S rDNA部分序列分析表明，该菌株与水稻黄单胞菌（Flavimonasoryzihabitans）的同源性为99.1%。它不仅能够降解不同的脂肪烃，但它也是对瓦斯油和十六烷的趋化性，如通过使用三种不同的趋化性方法，如琼脂糖塞和毛细管测定和群板分析所证明的。此外，该菌株对作为生长底物的各种碳源具有趋化性，包括葡萄糖、阿拉伯糖、甘露醇、甘油、葡萄糖酸盐、乙酸盐、琥珀酸盐、柠檬酸盐、苹果酸盐、乳酸盐和酪蛋白氨基酸。这是第一个报告的碳氢化合物降解细菌对纯烷烃，如hexadecane.The事实，环境分离物显示对污染物在现场的分离表明，趋化性可能会促进降解微生物之间的接触和它的底物的生物降解的趋化性。
In this report we describe the isolation of a strain from soil contaminated with gas oil by taking bacteria from a chemotactic ring on gas oil-containing soft agar plates. Partial 16 S rDNA sequencing of the isolated strain showed 99.1% identity with Flavimonas oryzihabitans. It was not only able to degrade different aliphatic hydrocarbons but it was also chemotactic towards gas oil and hexadecane, as demonstrated by the use of three different chemotaxis methods, such as agarose plug and capillary assays and swarm plate analysis. In addition, the strain was chemotactic to a variety of carbon sources that serve as growth substrates, including glucose, arabinose, mannitol, glycerol, gluconate, acetate, succinate, citrate, malate, lactate and casaminoacids. This is the first report on chemotaxis of a hydrocarbon-degrading bacterium towards a pure alkane, such as hexadecane.The fact that environmental isolates show chemotaxis towards contaminantts present in the site of isolation suggests that chemotaxis might enhance biodegradation by favouring contact between the degrading microorganism and its substrate.