CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro
CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro
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CAPE促进人脐带血来源的造血干细胞和祖细胞的体外扩增
DOI：
10.1007/s11427-014-4611-8
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发表时间：
2014-02-01
影响因子：
9.1
通讯作者：
Pei XueTao
中科院分区：
文献类型：
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作者：
Liu YiMing;Zhang BoWen;Pei XueTao
由于单份脐带血（UCB）中可收集的干细胞数量较少，其最初的用途仅限于儿科治疗。如果有一种培养方法能够在保持造血干细胞和祖细胞（HSPCs）自我更新能力的同时有效地扩增它们，那么脐带血HSPCs的临床应用将变得可行。近年来，人们进行了大量尝试在体外扩增人脐带血HSPCs。在这项研究中，我们报道咖啡酸苯乙酯（CAPE），一种来自蜜蜂提取物的小分子，能够促进HSPCs的体外扩增。用CAPE处理增加了培养的单核细胞中HSPCs的百分比。重要的是，用CAPE培养CD34(+) HSPCs导致总集落形成单位和高增殖潜能集落形成单位显著增加。爆式红系集落形成单位是受影响最大的集落类型，与对照组相比，在1μg/mL的CAPE处理组中增加了3.7倍以上。CAPE似乎通过上调干细胞因子（SCF）和缺氧诱导因子1 -α（HIF1 -α）的表达来诱导HSPC扩增。我们的数据表明，CAPE可能成为体外HSPC扩增的一种有效的培养基补充剂。
Due to the low number of collectable stem cells from single umbilical cord blood (UCB) unit, their initial uses were limited to pediatric therapies. Clinical applications of UCB hematopoietic stem and progenitor cells (HSPCs) would become feasible if there were a culture method that can effectively expand HSPCs while maintaining their self-renewal capacity. In recent years, numerous attempts have been made to expand human UCB HSPCs in vitro. In this study, we report that caffeic acid phenethyl ester (CAPE), a small molecule from honeybee extract, can promote in vitro expansion of HSPCs. Treatment with CAPE increased the percentage of HSPCs in cultured mononuclear cells. Importantly, culture of CD34(+) HSPCs with CAPE resulted in a significant increase in total colony-forming units and high proliferative potential colony-forming units. Burst-forming unit-erythroid was the mostly affected colony type, which increased more than 3.7-fold in 1 μg mL(-1) CAPE treatment group when compared to the controls. CAPE appears to induce HSPC expansion by upregulating the expression of SCF and HIF1-α. Our data suggest that CAPE may become a potent medium supplement for in vitro HSPC expansion.