PRESENCE OF PROTEIN PHOSPHATASE TYPE-1 AND ITS INVOLVEMENT IN TEMPERATURE-DEPENDENT FLAGELLAR MOVEMENT OF FOWL SPERMATOZOA
PRESENCE OF PROTEIN PHOSPHATASE TYPE-1 AND ITS INVOLVEMENT IN TEMPERATURE-DEPENDENT FLAGELLAR MOVEMENT OF FOWL SPERMATOZOA
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DOI：
10.1016/0014-5793(94)00752-7
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发表时间：
1994-08-15
期刊：
影响因子：
3.5
通讯作者：
TSUZUKI, Y
中科院分区：
文献类型：
--
作者：
ASHIZAWA, K;WISHART, GJ;TSUZUKI, Y
即使在ATP存在的情况下，在40摄氏度的禽类体温下，去膜的禽类精子的运动性也是可以忽略的。通过添加calyculin A、冈田酸、磷酸酶1型（PP 1）和PP-2A的特异性抑制剂以及蛋白磷酸酶1型（PP 1）的特异性抑制剂1或抑制剂2，可以恢复运动性。脱膜精子，刺激calyculin A或冈田酸，失去了他们的运动后，加入1 mM的氯化钙，但这是逐步恢复EGTA。使用PP 1的抗体对精子提取物进行免疫印迹，发现了一种主要的36-37 kDa的交叉反应蛋白，其对应于PP 1的已知催化亚基的分子量。这些结果表明，PP 1存在于家禽精子轴丝可能参与抑制家禽精子活力在40摄氏度通过Ca 2+依赖的调节系统。
Even in the presence of ATP, the motility of demembranated fowl spermatozoa was negligible at the avian body temperature of 40 degrees C. Motility could be restored by the addition of calyculin A, okadaic acid, specific inhibitors of phosphatase type 1 (PP1) and PP-2A, and inhibitor 1 or inhibitor 2, which are specific inhibitors of protein phosphatase type 1 (PP1). Demembranated spermatozoa, stimulated by calyculin A or okadaic acid, lost their motility following the addition of 1 mM CaCl2, but this was restored gradually by the stepwise addition of EGTA. Immunoblotting of sperm extract using an antibody to PP1 revealed a major cross-reacting protein of 36-37 kDa, which corresponded to the molecular weight of the known catalytic subunit of PP1. These results suggest that PP1 present in the fowl sperm axoneme may be involved in the inhibition of fowl sperm motility at 40 degrees C via Ca2+-dependent regulatory systems.