Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum)
Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum)
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DOI：
10.1046/j.1365-2761.2002.00375.x
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发表时间：
2002-06-01
影响因子：
2.5
通讯作者：
Austin, B
中科院分区：
文献类型：
--
作者：
Irianto, A;Austin, B
从大西洋鲑、萨尔莫、虹鳟、虹鳟和大菱鲆的肠道内容物中分离到需氧异养菌，其中11株（占总数的6%）对杀鲑气单胞菌有拮抗作用。其中4个培养物初步鉴定为A.结果表明，在单独投喂或等量混合投喂的条件下，对鱼类有益的微生物主要有弧菌属、河流弧菌属、肉杆菌属和一种未鉴定的革兰氏阳性球菌。补充有推定的益生菌的饲料表明生物体在胃肠道中存活7天。用益生菌喂养7天和14天导致A.杀鲑鱼的。没有迹象表明血清或粘液抗体A。但红细胞、巨噬细胞、淋巴细胞和白细胞数量增加，溶菌酶活性增强。
Aerobic heterotrophic bacteria were isolated from the intestinal contents of Atlantic salmon, Salmo salar, rainbow trout, Oncorhynchus mykiss, and turbot, Scophthalmus maximus, on tryptone soya agar and De Man Rogosa and Sharpe agar, of which 11 of 177 (6% of the total) of the isolates were antagonistic to Aeromonas salmonicida. Four of these cultures, which were identified tentatively as A. hydrophila, Vibrio fluvialis, Carnobacterium sp. and an unidentified Gram-positive coccus, were beneficial to fish when fed singly or as an equi-mixture. Feed supplemented with the putative probiotics indicated survival of the organisms in the gastrointestinal tract for 7 days. Feeding with the probiotics for 7 and 14 days led to better survival following challenge with A. salmonicida. There was no indication of serum or mucus antibodies to A. salmonicida, but there was an increased number of erythrocytes, macrophages, lymphocytes and leucocytes, and enhanced lysozyme activity in the fish.