Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients
Distribution of Porphyromonas gingivalis strains with fimA genotypes in periodontitis patients
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DOI：
10.1128/jcm.37.5.1426-1430.1999
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发表时间：
1999-05-01
影响因子：
9.4
通讯作者：
Hamada, S
中科院分区：
文献类型：
--
作者：
Amano, A;Nakagawa, I;Hamada, S
牙龈卟啉单胞菌（Porphyromonas gingivalis）的菌毛（Fimbriae，FimA）是牙龈卟啉单胞菌（Porphyromonas gingivalis）细胞表面的丝状组分，在牙周组织的定植和侵袭中起重要作用。根据fimA基因的核苷酸序列，我们以前证明fimA可以分为四种变体（I至IV型）。在本研究中，我们试图检测四个不同的fimA基因从牙周炎患者的唾液和牙菌斑样本分离使用PCR方法。设计了四组fimA型特异性引物用于PCR测定。这些引物选择性扩增fimA基因的392-bp（I型）、257-bp（II型）、247-bp（III型）和251-bp（IV型）DNA片段。用牙龈卟啉单胞菌的参考菌株以类型特异性方式观察到阳性PCR结果。口腔和非口腔细菌的所有其他实验室菌株保存阴性结果。fimA型特异性检测的PCR测定的灵敏度在5至50个牙龈卟啉单胞菌细胞之间。临床样本从93例牙周炎患者的深袋（大于或等于4 mm）的唾液和龈下菌斑中获得。从标本中提取细菌基因组DNA，用PCR扩增目的片段，73例患者中检出牙龈卟啉单胞菌牙龈卟啉单胞菌fimA基因型分布为：Ⅰ型5.4%，Ⅱ型58.9%，Ⅲ型6.8%，Ⅲ型10.5%，Ⅳ型10.5%。IV型占12.3%，I型和II型占6.8%，II型和IV型占2.7%，无法分型占6.8%。牙龈卟啉单胞菌Ⅱ型fimA在深牙周袋中检出率较高，浅牙周袋（4 mm）与深牙周袋（≥ 8 mm）的发生率差异有统计学意义。这些结果表明具有II型fimA的牙龈卟啉单胞菌菌株。在牙周炎患者中明显更占优势，我们推测这些微生物参与了牙周病的破坏性进展。
Fimbriae (FimA) of Porphyromonas gingivalis are filamentous components on the cell surface and are thought to play an important role in the colonization and invasion of periodontal tissues. We previously demonstrated that fimA can be classified into four variants (types I to IV) On the basis of the nucleotide sequences of the fimA gene. In the present study, we attempted to detect the four different fimA genes in saliva and plaque samples isolated from patients with periodontitis using the PCR method. Four sets of fimA type-specific primers were designed for the PCR assay. These primers selectively amplified 392-bp (type I), 257-bp (type II), 247-bp (type III), and 251-bp (type IV) DNA fragments of the fimA gene. Positive PCR results were observed with reference strains of P. gingivalis in a type-specific manner. All other laboratory strains of oral and nonoral bacteria Save negative results. The sensitivity of the PCR assay for fimA type-specific detection was between 5 and 50 cells of P, gingivalis. Clinical samples were obtained from saliva and subgingival plaque from deep pockets (greater than or equal to 4 mm) of 93 patients with periodontitis. Bacterial genomic DNA was isolated from the samples, and the targeted fragments were amplified by PCR The presence of P. gingivalis was demonstrated in 73 patients (78.5%), and a single fimA gene Has detected in most patients, The distribution of the four fimA types among the P. gingivalis-positive patients was as follows: type I, 5.4%; type II, 58.9%; type III, 6.8%; type IV, 12.3%; types I and II, 6.8%; types II and IV: 2.7%; and untypeable, 6.8%. P, gingivalis with type II fimA was detected more frequently in the deeper pockets, and a significant difference of the occurrence was observed between shallow (4 mm) and deep (greater than or equal to 8 mm) pockets. These results suggest that P. gingivalis strains that possess type II fimA. are significantly more predominant in periodontitis patients, and we speculate that these organisms are involved in the destructive progression of periodontal diseases.