NONINVASIVE IMAGING OF LIVING HUMAN SKIN WITH DUAL-WAVELENGTH OPTICAL COHERENCE TOMOGRAPHY IN TWO AND THREE DIMENSIONS
NONINVASIVE IMAGING OF LIVING HUMAN SKIN WITH DUAL-WAVELENGTH OPTICAL COHERENCE TOMOGRAPHY IN TWO AND THREE DIMENSIONS
复制标题
DOI：
10.1117/1.429897
复制
发表时间：
1998-10-01
影响因子：
3.5
通讯作者：
Farkas, Daniel L.
中科院分区：
文献类型：
--
作者：
Pan, Yingtian;Farkas, Daniel L.
我们展示了光学相干域层析成像(OCT)在近红外范围内的两个波长(830和1285 nm)同时对活体皮肤进行无创成像的潜力。描述了提供生物组织的快速二维(2D)和三维(3D)成像的单模光纤相干层析扫描仪的原型的技术细节。讨论了仪器参数和活体组织的动态特性对图像对比度和分辨率的影响以及对斑点噪声抑制的影响。通过比较单次扫描的OCT图像和相应的帧平均OCT图像，研究了成像速度对OCT图像质量的影响，后者降低了斑点噪声并丢失了一些细微的结构。理论预测和人体皮肤成像的实验结果都表明，较长的波长可以减小多次散射对图像对比度和分辨率的影响，从而使OCT成像的有效穿透深度增加到2 mm左右。给出了一些活体皮肤显微解剖结构的高分辨率2D和3D图像，并对其进行了分析，说明了OCT在深入、非侵入性地显示活体皮肤微观形态方面的独特能力。(C)1998年光学仪器工程师学会。
We demonstrate the potential of optical coherence-domain tomography (OCT) for noninvasive imaging of living skin simultaneously at two wavelengths in the near infrared range (830 and 1285 nm). The technical details of a prototype monomode fiber-optic coherence tomographic scanner providing rapid two-dimensional (2D) and three-dimensional (3D) imaging of biological tissues are described. The effects of both instrumentation parameters and the dynamic characteristics of living tissue on image contrast and resolution and on speckle reduction are discussed. The impact of imaging speed on OCT image quality is studied by a comparison between a single scan and the corresponding frame-averaged OCT images, with the latter resulting in decreased speckle noise as well as loss of some subtle structures. Both theoretical predictions and experimental results in human skin imaging show that longer wavelength can minimize the influence of multiple scattering on image contrast and resolution and thus increase the effective penetration depth of OCT imaging to about 2 mm. Some high-resolution 2D and 3D images of microscopic anatomic structures of living human skin are presented and analyzed, illustrating the unique capability of OCT for in depth, noninvasive visualization of living skin microscopic morphology in vivo. (C) 1998 Society of Photo-Optical Instrumentation Engineers.