Embryonic stem cell lines derived from human blastocysts
Embryonic stem cell lines derived from human blastocysts
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DOI：
10.1126/science.282.5391.1145
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发表时间：
1998-11-06
期刊：
影响因子：
56.9
通讯作者：
Jones, JM
中科院分区：
文献类型：
--
作者：
Thomson, JA;Itskovitz-Eldor, J;Jones, JM
描述了人类胚泡衍生的多能细胞系，具有正常的核型，表达高水平的端粒酶活性以及表达表征灵长类动物胚胎干细胞但没有表征其他早期谱系的细胞表面标记。在体外未分化的增殖4到5个月后，这些细胞仍然保持了形成滋养细胞和衍生物的发育潜力，包括所有三个胚胎细菌层的衍生物，包括肠道上皮（内胚层）；软骨，骨骼，平滑肌和横纹肌（中胚层）；和神经上皮，胚胎神经节和分层的鳞状上皮（外胚层）。这些细胞系应在人类发育生物学，药物发现和移植医学中有用。
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.