Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f
Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f
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DOI：
10.1016/j.bbabio.2012.02.026
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Chen, Min
中科院分区：
文献类型：
--
作者：
Li, Yaqiong;Scales, Nicholas;Chen, Min
叶绿素f和叶绿素d都是光合生物中的红移叶绿素，它们将光子吸收扩展到近红外区域。这扩大了可用于驱动光合作用的光的范围。叶绿素的定量测定是光生物学和光化学领域研究叶绿素-光合作用反应的关键步骤。还没有研究出叶绿素f的定量测定方法。尽管叶绿素d在1943年被发现，但目前还没有一种方法可以精确地定量测定叶绿素d。为了获得叶绿素f和叶绿素d的消光系数（λ），根据每个叶绿素分子含有一个镁（Mg）原子的事实，通过电感耦合等离子体质谱法测定叶绿素的浓度。在100%甲醇中，摩尔消光系数λ（chl）（f）为71.11 x 10（3）L mol（-1）A（707）（nm）cm（-1），λ（chl）（d）为63.68 x 10（3）L mol（-1）A（697）（nm）cm（-1）。这篇文章是特刊的一部分，题为：光合作用研究的可持续性：从自然到人工。(C)2012 Elsevier B. V.保留所有权利。
Both chlorophyll f and chlorophyll d are red-shifted chlorophylls in oxygenic photosynthetic organisms, which extend photon absorbance into the near infrared region. This expands the range of light that can be used to drive photosynthesis. Quantitative determination of chlorophylls is a crucial step in the investigation of chlorophyll-photosynthetic reactions in the field of photobiology and photochemistry. No methods have yet been worked out for the quantitative determination of chlorophyll f. There is also no method available for the precise quantitative determination of chlorophyll d although it was discovered in 1943. In order to obtain the extinction coefficients (epsilon) of chlorophyll f and Chlorophyll d, the concentrations of chlorophylls were determined by Inductive Coupled Plasma Mass Spectrometry according to the fact that each chlorophyll molecule contains one magnesium (Mg) atom. Molar extinction coefficient epsilon(chl) (f) is 71.11 x 10(3) L mol(-1) A(707) (nm) cm(-1) and epsilon(chl) (d) is 63.68 x 10(3) L mol(-1) A(697) (nm) cm(-1) in 100% Methanol. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial. (C) 2012 Elsevier B.V. All rights reserved.