Laser-induced micro-bubbles in cells
Laser-induced micro-bubbles in cells
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DOI：
10.1016/j.ijheatmasstransfer.2004.08.012
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发表时间：
2005-01-01
影响因子：
5.2
通讯作者：
Lukianova, E
中科院分区：
文献类型：
--
作者：
Lapotko, D;Lukianova, E
我们报告了单个活细胞和有限微体积溶液中激光诱导微气泡的实验研究和检测。使用脉冲激光辐射（532nm，10ns）作为局部加热源，我们检测到光吸收介质中的微气泡至少是由两种机制引起的：将介质加热到临界温度以上（超过 300 摄氏度）（称为“热”气泡）和在低得多的温度（30-150 摄氏度）下稀疏压力波的作用（称为“冷”气泡）。采用两种光热 (PT) 方法（热透镜和 PT 时间分辨成像）对人红细胞、血红蛋白均匀溶液、中性红染料和聚苯乙烯纳米粒子悬浮液的气泡产生阈值、气泡形成概率和气泡寿命进行了实验研究。 (C) 2004 Elsevier Ltd. 保留所有权利。
We report experimental study and detection of laser-induced micro-bubbles in individual live cells and in confined micro-volumes of solutions. Using pulsed laser radiation (532nm, 10ns) as the source of local heating we detected micro-bubbles in light-absorbing media that were caused at least by two mechanisms-heating of the media above (more than 300degreesC) critical temperature (referred as 'hot' bubbles) and action of rarefaction pressure waves (referred as 'cold' bubbles) at much lower temperatures (30-150degreesC). Bubble generation thresholds, probabilities of bubble forming and bubble lifetimes were experimentally studied with two photothermal (PT) methods-thermal lensing and PT-time-resolved imaging for human red blood cells, homogeneous solutions of haemoglobin, neutral red dye and for suspension of polystyrene nano-particles. (C) 2004 Elsevier Ltd. All rights reserved.