Structural characterization and anti-osteoporosis effect of an arabinomannan from Anemarrhena asphodeloides Bge.
Structural characterization and anti-osteoporosis effect of an arabinomannan from Anemarrhena asphodeloides Bge.
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DOI：
10.1016/j.ijbiomac.2023.123324
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发表时间：
2023-01
影响因子：
8.2
通讯作者：
Bingfeng Lin;Xuehui Deng;Pingcui Xu;Qitao Ye;Guizhi Zhao;Mingli Ye;Nani Wang
中科院分区：
文献类型：
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作者：
Bingfeng Lin;Xuehui Deng;Pingcui Xu;Qitao Ye;Guizhi Zhao;Mingli Ye;Nani Wang
为了发现具有抗糖尿病骨质疏松（DOP）活性的多糖，并明确其结构，从阿玛霉（Anemarrhena aspholoides Bge）中分离得到分子量为14.0 kDa的阿拉伯甘露聚糖（PAAP-1B）。采用柱层析法。它由阿拉伯糖、甘露糖和半乳糖组成，摩尔比为6:3:1。PAAP-1B的主链由1,5-α-Araf、1,4-β-Manp和1,6-β-Galpresidues组成，它们在α- arafes和β-Galpresidues的C3处支链。侧链分别为T-α-Araf、T-α-Manp、T-β-Galp和1,6-β-Galp。PAAP-1B可减轻四氧嘧啶诱导小鼠股骨和胫骨的DOP和铁下垂。它还通过降低4-羟基壬烯醛、丙二醛、线粒体活性氧化物种水平和脂质过氧化，同时逆转溶质载体家族7、膜11和谷胱甘肽表达的下调，抑制晚期糖基化终产物诱导的成骨细胞的铁凋亡。
To discover the polysaccharide with anti-diabetic osteoporosis (DOP) activity and clarify its structure, an arabinomannan (PAAP-1B) with a molecular weight of 14.0 kDa was isolated from Anemarrhena asphodeloides Bge. using column chromatography. It consists of arabinose, mannose, and galactose in a molar ratio of 6:3:1. PAAP-1B has a backbone composed of 1,5-α-Araf, 1,4-β-Manp, and 1,6-β-Galpresidues that are branched at C3 of α-Arafand β-Galpresidues. The side chains are T-α-Araf, T-α-Manp, T-β-Galp, and 1,6-β-Galp. PAAP-1B attenuated DOP and reduced ferroptosis in the femurs and tibias of alloxan-induced mice. It also suppressed ferroptosis in advanced glycation end product-induced osteoblasts by decreasing 4-hydroxynonenal, malondialdehyde, mitochondrial reactive oxidative species levels, and lipid peroxidation, while reversing the downregulation of solute carrier family 7 membrane 11 and glutathione expression.