The role of CD98hc in mouse macrophage functions
The role of CD98hc in mouse macrophage functions
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DOI：
10.1016/j.cellimm.2012.04.012
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发表时间：
2012-03-01
影响因子：
4.3
通讯作者：
Ito, Yasuhiko
中科院分区：
文献类型：
--
作者：
Tsumura, Hideki;Ito, Morihiro;Ito, Yasuhiko
CD98hc 是一种 II 型跨膜蛋白，与几种 L 型氨基酸转运蛋白之一共价连接。 CD98hc 最初被鉴定为淋巴细胞活化标记物。在本研究中，我们使用组织特异性敲除小鼠 CD98hc（CD98hc(flox/-)LysM-cre 小鼠）检查了 CD98hc 在巨噬细胞功能中所起的作用。当分离的腹腔巨噬细胞孵育48小时时，从敲除小鼠中获得的巨噬细胞呈现圆形形态，而从对照小鼠中获得的几乎所有细胞都是纺锤形的。据报道，CD98hc 缺陷型腹膜巨噬细胞的巨噬细胞功能（例如抗原呈递、吞噬和融合活性）降低。此外，当用IFN-γ/LPS或IL-4刺激CD98hc缺陷型巨噬细胞时，与对照巨噬细胞中观察到的相比，NO2或精氨酸酶-I的产生减少。这些发现表明CD98hc分子在巨噬细胞的激活和功能中发挥重要作用。 (C) 2012 Elsevier Inc. 保留所有权利。
CD98hc is a type II transmembrane protein that covalently links to one of several L-type amino acid transporters. CD98hc was first identified as a lymphocyte activation marker. In this study, we examined the role that CD98hc plays in the functions of macrophages using tissue specific knock-out miceCD98hc (CD98hc(flox/-)LysM-cre mice). When isolated peritoneal macrophages were incubated for 48 h, the macrophages obtained from the knock-out mice showed round-shaped morphologies, while almost all of the cells obtained from the control mice were spindle-shaped. The macrophage functions such as the antigen-presenting, phagocytic, and fusion activities, have been reported to decrease in CD98hc-deficient peritoneal macrophages. In addition, when the CD98hc deficient macrophages were stimulated with either IFN-gamma/LPS or IL-4, the production of NO2 or arginase-I decreased in comparison to that observed in the control macrophages. These findings show that the CD98hc molecules play an important role in the activation and functions of macrophages. (C) 2012 Elsevier Inc. All rights reserved.