Effects of inositol starvation on phospholipid and glycan syntheses in Saccharomyces cerevisiae
Effects of inositol starvation on phospholipid and glycan syntheses in Saccharomyces cerevisiae
复制标题
肌醇饥饿对酿酒酵母磷脂和聚糖合成的影响
DOI：
10.1128/jb.142.1.79-89.1980
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发表时间：
1980
影响因子：
3.2
通讯作者：
R. Lester
中科院分区：
文献类型：
--
作者：
B. Hanson;R. Lester
研究了肌醇饥饿对酿酒酵母肌醇营养缺陷菌的早期生化影响，以此来确定肌醇的细胞作用。当肌醇停用时，~(32)P标记的无机磷酸盐进入磷脂酰肌醇和含磷脂酰肌醇的鞘磷脂的参入率立即分别下降80%和50%，而其他主要磷脂的合成在控制速率下持续2~3h。[U-14C]葡萄糖掺入细胞壁葡聚糖在饥饿后立即开始下降，甘露聚糖在饥饿后80min降至初始速率的50%，酸、碱不溶性葡聚糖在饥饿后140min降至初始速率的50%。这些细胞壁多糖和磷脂酰肌醇合成速率的变化是肌醇饥饿的最早影响，通过将放射性前体掺入三氯乙酸不溶细胞物质来衡量蛋白质和核糖核酸的合成受到抑制。这些结果表明，磷脂酰肌醇可能在酵母多糖的合成或分泌中起直接作用。
The early biochemical consequences of inositol starvation in an inositol auxotroph of Saccharomyces cerevisiae were examined as a means of determining the cellular role of inositol. Upon withdrawal of inositol, the rate of incorporation of 32P-labeled inorganic phosphate into phosphatidylinositol and into the phosphoinositol-containing sphingolipids immediately dropped by 80 and 50%, respectively; however, synthesis of the other major phospholipids continued for 2 to 3 h at control rates. The incorporation of [U-14C]glucose into cell wall glycans began to decline immediately poststarvation and decreased to 50% of the initial rate by 80 min for mannan and by 140 min for alkali- and acid-insoluble glucan. These changes in the rates of synthesis of cell wall glycan and phosphatidylinositol were the earliest effects of inositol starvation, preceding inhibition of the synthesis of protein and ribonucleic acid as measured by incorporation of radioactive precursors into trichloroacetic acid-insoluble cell material. These results suggest that phosphatidylinositol may play a direct role in the synthesis or secretion of yeast glycans.