Full-length transcriptome analysis of Misgurnus anguillicaudatus
Full-length transcriptome analysis of Misgurnus anguillicaudatus
复制标题
泥鳅全长转录组分析
DOI：
10.1016/j.margen.2020.100785
复制
发表时间：
2020-12-01
期刊：
影响因子：
1.9
通讯作者：
Du, Zongjun
中科院分区：
文献类型：
--
作者：
Luo, Wei;Wu, Qing;Du, Zongjun
在没有参考基因组的物种中，转录本的忠实分析是基因发现、基因组表征和注释过程的关键组成部分。本研究使用Pacific Bioscience（PacBio）单分子实时（SMRT）测序系统生成了28，001个泥鳅全长转录本。共鉴定出77，346个共有异构体，并检测到18，991个完整的开放阅读框架（ORF）。总共鉴定了1345种lncRNA，具有高置信度，另外还鉴定了一些众所周知的转录因子。本研究可能有助于进一步探索M.鳗尾鱼
Faithful analysis of transcripts represents a critical component of the gene discovery, genomic characterization and annotation process in species without a reference genome. The present study generated 28,001 full-length transcripts of Misgurnus anguillicaudatus using the Pacific Bioscience (PacBio) single-molecule real-time (SMRT) sequencing system. A total of 77,346 consensus isoforms were identified, and 18,991 complete open reading frames (ORFs) detected. In total, 1345 lncRNAs were identified with high-confidence, with additional identification of a number of well-known transcription factors. The present study may facilitate additional exploration of the genomic signatures of M. anguillicaudatus.