Inhalation of diesel exhaust enhances antigen-specific IgE antibody production in mice
Inhalation of diesel exhaust enhances antigen-specific IgE antibody production in mice
复制标题
DOI：
10.1016/s0300-483x(96)03539-1
复制
发表时间：
1997-01-15
期刊：
影响因子：
4.5
通讯作者：
Endo, T
中科院分区：
文献类型：
--
作者：
Fujimaki, H;Saneyoshi, K;Endo, T
为研究柴油机尾气（DE）吸入对小鼠IgE抗体产生的影响，BALB/c小鼠分别吸入0（对照组）、3.0和6.0 mg/m3的DE 3周。在吸入DE前即刻、吸入DE后即刻和吸入DE后3周，用卵清蛋白（OA）进行鼻内致敏，间隔3周，共3次。接触DE的小鼠和对照组小鼠的体重和胸腺重量基本相同，但接触6 mg/m3的小鼠的脾脏重量显著增加。暴露于6 mg/m3的小鼠血清中的抗OA IgE抗体滴度显著高于对照组。DE暴露小鼠和对照小鼠血清中总IgE和抗OA IgG基本保持不变。为了研究暴露于6 mg/m（3）的小鼠中细胞因子的产生，在体外用OA刺激DE暴露小鼠和对照小鼠的脾细胞，并通过ELISA测量培养物上清液中细胞因子的产生。与对照组相比，暴露小鼠脾细胞中体外抗原刺激的白细胞介素-4（IL-4）和白细胞介素-10（IL-10）的产生显著增加。在体外干扰素（IFN）-γ的生产在脾细胞暴露小鼠显着下降。因此，DE吸入显示通过改变细胞因子网络对小鼠中抗原特异性IgE抗体产生具有不利影响。版权所有（C）1997 Elsevier Science爱尔兰有限公司
To examine the effects of diesel exhaust (DE) inhalation on IgE antibody production, BALB/c mice were exposed to 0 (control), 3.0 and 6.0 mg/m(3) DE inhalation for 3 weeks. Intranasal sensitization with ovalbumin (OA) three times at intervals of 3 weeks was conducted immediately before, immediately after and 3 weeks after DE inhalation. Body weight and thymus weight for the DE-exposed and control mice were essentially the same but spleen weight in mice exposed to 6 mg/m(3) significantly increased. Anti-OA IgE antibody titers in the sera of mice exposed to 6 mg/m(3) was significantly higher than the control. Total IgE and anti-OA IgG in sera for DE-exposed and control mice remained basically the same. To investigate cytokine production in mice exposed to 6 mg/m(3), spleen cells from DE-exposed and control mice were stimulated with OA in vitro and cytokine production in the culture supernatants was measured by ELISA. In vitro antigen-stimulated interleukin-il (IL-4) and -10 (IL-10) production in spleen cells of exposed mice significantly increased compared to the control. In vitro interferon (IFN)-gamma production in spleen cells of exposed mice markedly decreased. DE inhalation is thus shown to have adverse effect on antigen-specific IgE antibody production in mice through alteration of the cytokine network. Copyright (C) 1997 Elsevier Science Ireland Ltd.