Concentration- and composition-dependent effects of metal ions on human MG-63 osteoblasts
Concentration- and composition-dependent effects of metal ions on human MG-63 osteoblasts
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DOI：
10.1002/jbm.10106
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发表时间：
2002-06-05
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影响因子：
--
通讯作者：
Jacobs, JJ
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文献类型：
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作者：
Hallab, NJ;Vermes, C;Jacobs, JJ
来自植入物的金属碎片已被证明会改变细胞培养中成骨细胞的功能。然而，其仍然不清楚，如果特定形式的离子金属释放参与假体周围骨溶解的发病机制。我们通过用8种浓度（0.001-10.0 mM）的Cr+3、Mo+5、Al+3、Ta+5、Co+2、Ni+2、Fe+3、Cu+2、Mn+2、Mg+2、Na+2和V+3氯化物溶液处理人成骨细胞样MG-63骨肉瘤细胞，评估植入金属离子形式的相对影响。结果表明，不同的金属离子影响成骨细胞增殖，活力，I型胶原基因的表达，和细胞因子的释放。金属离子按毒性从最小到最大的顺序（基于存活率降低50）排列如下：Na < Cr < Mg < Mo < Al < Ta < Co < Ni < Fe < Cu < Mn <V。金属诱导的成骨细胞增殖的降低在排列上是相似的。无毒浓度的金属对前胶原α 1 [1]基因表达没有影响;只有在毒性浓度下，金属才能降低基因表达。最有毒的金属（V、Mn、Fe和Ni）也是发现在每个细胞基础上诱导IL-6分泌的唯一金属（在测试的细胞因子中，白细胞介素6（IL-6）、白细胞介素β 1（IL-1 β），转化生长因子β I（TGF-β 1）和肿瘤坏死因子α（TNF-α），对于任何浓度的任何金属，48小时后在培养基中仅可检测到IL-6）。毒性较低的金属（例如，Co和Cr）对IL-6释放的影响很小，即使在高浓度下也是如此。一般来说，金属离子降低成骨细胞功能（即，增殖和胶原基因表达）与毒性程度成比例。这些结果支持以下假设：与植入金属器械的金属碎屑相关的不良局部细胞反应（尤其是坏死反应）可能部分是由于植入物或颗粒碎屑释放的金属离子。(C)2002 Wiley Periodicals，Inc.
Metal debris from implants has been shown to alter the function of osteoblasts in cell cultures. Its remains unclear, however, if specific forms of released ionic metals are involved in the pathogenesis of periprosthetic osteolysis. We evaluated the relative effects of ionic forms of implant metals by treating human osteoblast-like MG-63 osteosarcoma cells with eight concentrations (0.001-10.0 mM) of Cr+3, Mo+5, Al+3, Ta+5, Co+2, Ni+2, Fe+3, Cu+2, Mn+2 Mg+2, Na+2, and V+3 chloride solutions. The results demonstrated that the metal ions differentially affected osteoblast proliferation, viability, type-I collagen gene expression, and cytokine release. The metal ions were ranked in order from least to most toxic (based on a 50 reduction in viability) as follows: Na < Cr < Mg < Mo < Al < Ta < Co < Ni < Fe < Cu < Mn < V. Metal-induced decreases in osteoblast proliferation were similar in ranking. Nontoxic concentrations of metals had no effect on procollagen alpha1[1] gene expression; only at toxic concentrations did metals produce a decrease in gene expression. The most toxic metals (V, Mn, Fe, and Ni) were also the only metals found to induce IL-6 secretion on a per cell basis (of the cytokines tested, interleukin 6 (IL-6), interleukin beta 1 (IL-1beta), transforming growth factor beta I (TGF-beta1), and tumor necrosis factor alpha (TNF-alpha), only IL-6 was detectable in the culture medium after 48 h for any metal at any concentration). Less toxic metals (e.g., Co and Cr) had little effect on IL-6 release, even at high concentrations. In general, metal ions reduced osteoblast function (i.e., proliferation and collagen gene expression) in proportion to the degree of toxicity. These results support the hypothesis that adverse local cellular responses (particularly necrotic responses) associated with metal debris from implanted metallic devices may be due in part to metal ions released from implants or from particulate debris. (C) 2002 Wiley Periodicals, Inc.